Human Apolipoprotein AI ELISA Kit (APOA1) (ab108803)


  • 製品名
    Human Apolipoprotein AI ELISA Kit (APOA1)
    Apolipoprotein A I キット 製品一覧
  • 検出方法
  • 再現性
    サンプル N 平均値 SD CV%
    Overall 4%
    サンプル N 平均値 SD CV%
    Overall 10.1%
  • サンプルの種類
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Cerebral Spinal Fluid
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    = 3 ng/ml
  • 検出範囲
    6.25 ng/ml - 100 ng/ml
  • 添加回収試験

    97 %

  • 全工程の試験時間
    4h 00m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Apolipoprotein AI (APOA1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein AI concentrations in plasma, serum, urine, saliva, milk, CSF and cell culture samples.

    An Apolipoprotein AI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein AI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein AI captured in plate.

    Get results in 90 minutes with Human Apolipoprotein AI ELISA Kit (ab189576) from our SimpleStep ELISA® range.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム


  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Apolipoprotein AI Antibody 1 x 120µl
    Apolipoprotein AI Microplate (12 x 8 well strips) 1 unit
    Chromogen Substrate 1 x 8ml
    Human Apolipoprotein AI Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • 研究分野
  • 機能
    Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • 組織特異性
    Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease.
  • 関連疾患
    High density lipoprotein deficiency 2
    High density lipoprotein deficiency 1
    APOA1 mutations may be involved in the pathogenesis of amyloid polyneuropathy-nephropathy Iowa type, also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III (PubMed:3142462 and PubMed:2123470). The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis.
    Amyloidosis 8
  • 配列類似性
    Belongs to the apolipoprotein A1/A4/E family.
  • 翻訳後修飾
    Met-110 and Met-136 are oxidized to methionine sulfoxides.
    Phosphorylation sites are present in the extracellular medium.
  • 細胞内局在
  • Information by UniProt
  • 別名
    • Apo-AI
    • ApoA-I
    • APOA1
    • Apolipoprotein A-I
    • Apolipoprotein A-I(1-242)
    • Apolipoprotein A1
    • Truncated apolipoprotein A-I
    see all
  • 参照データベース



Our Abpromise guarantee covers the use of ab108803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.


  • APOA1 measured in various samples showing quantity (microgram) per mL of tested sample

  • Representative Standard Curve using ab108803



ab108803 has not yet been referenced specifically in any publications.

レビューと Q&A

Measure APOA1 in plasma samples

Excellent Excellent 5/5 (Ease of Use)
I measured APOA1 in plasma sample using ab108803 which should measure in cell culture supernatant, saliva, milk, urine, or cerebral spinal fluid. I tried to find the dilution factor for plasma samples. I found that 1:50000 is the optimum ratio for dilution and detection. Ultimately, this product can measure APOA1 in plasma samples.

Abcam user community

Verified customer

投稿 Dec 28 2016

The amount of standard for lot GR132237-1 = 3.2 ug (micrograms).

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The lab let me know that the kit did not work for Bovine samples.

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I can confirm that the main difference between these kits is that the ab108804 Apolipoprotein A1 Human ELISA Kit uses a competitive ELISA quantification, whereas ab108803 Apolipoprotein A I Human ELISA Kituses sta...

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I have also forwarded this information to the laboratory in order to investigate the problem together. The laboratory and I do agree that all steps have been performed as indicated and do not ...

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I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

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