製品の概要

  • 製品名Human Antithrombin III ELISA Kit
    Antithrombin III キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.8%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.1%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Cell culture media, Heparin Plasma, Cerebral Spinal Fluid
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 3 ng/ml
  • 検出範囲
    3.13 ng/ml - 200 ng/ml
  • 添加回収試験

    98.5 %

  • 全工程の試験時間
    4h 0m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Antithrombin III Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Antithrombin III in urine, milk, saliva, cerebrospinal fluid, plasma, serum, and cell culture supernatants.


    An antithrombin III specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an antithrombin III specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of antithrombin III captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • 組織特異性Found in plasma.
  • 関連疾患Antithrombin III deficiency
  • 配列類似性Belongs to the serpin family.
  • 翻訳後修飾Phosphorylation sites are present in the extracellular medium.
  • 細胞内局在Secreted > extracellular space.
  • Information by UniProt
  • 別名
    • ANT3_HUMAN
    • Antithrombin-III
    • ATIII
    • Serpin C1
    • SERPINC1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Antithrombin III ELISA Kit 画像

  • Linearity: Antithrombin III in samples at different diutions, corrected for the factor of dilution  (duplicates +/- SD)

  • Linearity: Antithrombin III in samples at different diutions, corrected for the factor of dilution (duplicates +/- SD), showing ng x mL-1 on y-axis.

  • Linearity: Antithrombin III in samples at different diutions, corrected for the factor of dilution  (duplicates +/- SD)

  • Antithrombin III in cell culture supernatants (duplicates +/- SD), tested in the dilution range of 1:1-1:100.

  • Antithrombin III in human biological fluids (duplicates +/- SD). The samples were differently diluted  (serum and Li-heparine plasma: 1e4-1e5; CSF: 1e2-1e3; milk: 10-100; saliva: 5-100; urine: neat)

  • Representative standard curve using ab108801.

プロトコール

Human Antithrombin III ELISA Kit (ab108801) 使用論文

ab108801 has not yet been referenced specifically in any publications.

Product Wall

Please find below the information on how to prepare the standard for lots GR116626-1, Lot GR115289-1 and Lot GR106377-1 of the Antithrombin III Human ELISA Kit ab108801:


· MIX Diluent Concentrate (10x): If crystals have formed in t...

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