製品の概要

  • 製品名
    Human anti-Annexin V ELISA Kit
    Annexin V キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 2%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 3.8%
  • サンプルの種類
    Cell culture supernatant
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    1.2 ng/ml
  • 検出範囲
    6.3 ng/ml - 400 ng/ml
  • 添加回収試験

    86 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 86 74% - 100%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s anti-Annexin V Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human anti-Annexin V antibody concentrations in cell culture supernatant.

    A 96-well plate has been precoated with Annexin V to which cognate antibodies bind. Standards or test samples are added to the wells and incubated. A horseradish peroxidase (HRP) labeled Annexin V conjugate is added to the wells, which is bound by the immobilized Annexin V-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of anti-Annexin V antibodies sample captured in the plate.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab119504.

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参考文献

ab119504 has not yet been referenced specifically in any publications.

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