製品の概要

  • 製品名
    Human Annexin V ELISA Kit
    Annexin V キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall < 10%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 11%
  • サンプルの種類
    Cell culture supernatant
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    0.33 ng/ml
  • 検出範囲
    0.78 ng/ml - 50 ng/ml
  • 添加回収試験

    85 %

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Annexin V Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Annexin V concentrations in cell culture supernatant and serum.

    Annexin V specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated Annexin V detection antibody then incubated at room temperature and washed. A Streptavidin-HRP conjugate is then added to each well, incubated at room temperature and washed. TMB is added and catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Annexin V sample captured in plate.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab119503.

プロトコール

参考文献

ab119503 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you very much for your inquiry.

Once the standard is reconstituted, we recommend using them immediately and then discarding the remaining portion.
We have not performed stability studies regarding the long -term storage of reconsti...

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Once the standard is reconstituted, we recommend using them immediately and then discarding the remaining portion.


We have not performed stability studies regarding the long -term storage of reconstituted standard protein so unfortunate...

Read More

Thank you for contacting us. The reconstitution instructions for the standard are lot specific and will be written on the vial. If for any reason it is not, please let us know your lot number and we will be happy to find that information for you.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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