製品の概要

  • 製品名Human Angiostatin ELISA Kit
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 20 ng/ml
  • 検出範囲
    31.25 ng/ml - 2000 ng/ml
  • 添加回収試験

    < 95 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 95.43 83% - 104%
    Serum 92.45 81% - 101%
    Plasma 93.31 82% - 102%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Angiostatin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiostatin in serum, plasma (heparin) and cell culture supernatants.

    This assay employs an antibody specific for Human Angiostatin coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiostatin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiostatin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiostatin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimisation may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Angiostatin is an internal proteolytic fragment of plasminogen, containing at least three Kringle domains. The Kringle domains have specific anti-migratory and anti-proliferative activities. Angiostatin, an endogenous angiogenesis inhibitor, inhibits endothelial cell proliferation in vitro and angiogenesis in vivo. It is a potent and specific natural inhibitor of angiogenisis and metastatic tumor growth.
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab99973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Angiostatin ELISA Kit 画像

  • Titration of NIBSC Plasmin (97/536), with background signal subtracted (duplicates; +/- SD).

  • Angiostatin measured in human undiluted biological fluids (duplicates +/- SD). Signals from mouse and rat (pooled) plasma and sera were below level of detection.

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Representative Standard Curve using ab99973.

プロトコール

Human Angiostatin ELISA Kit (ab99973) 使用論文

ab99973 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for your call last week and for your patience while I have been in touch with the lab regarding your question.
My contact at the lab has said that the plates are optimized with the provided wash buffer, but it is a PBS-based bu...

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The lab confirmed that the assay will detect plasminogen, in addition to angiostatin. I am going to ask that we change the name of the assay to anti - Angiostatin/Plasminogen, or at least add a note indicating reactivity with plasminogen. Thank you for...

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Thank you for contacting us.

The capture antibody used in this ELISA (ELH-Angiostatin-001) is a mouse monoclonal that recognizes the amino acid sequence of 98 – 358 of human Angiostatin Kringle 1 – 3. The detection antibody is a goat polyclo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"