製品の概要

  • 製品名
    Human Angiopoietin 2 ELISA Kit (ANG2)
    Angiopoietin 2 キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 10 pg/ml
  • 検出範囲
    4.12 pg/ml - 3000 pg/ml
  • 添加回収試験

    88 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 82.1 76% - 92%
    Serum 85.3 82% - 109%
    Plasma 96.9 95% - 116%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Angiopoietin 2 (ANG2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiopoietin 2 in serum, plasma and cell culture supernates.

    This assay employs an antibody specific for Human Angiopoietin 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiopoietin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimisation may be required with urine samples.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent 1 x 15ml
    Angiopoietin 2 Microplate (12 x 8 well strip) 1 unit
    Biotinylated anti-Human Angiopoietin 2 (lyophilized) 2 vials
    Recombinant Angiopoietin 2 Standard Human 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究分野
  • 機能
    Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
  • 配列類似性
    Contains 1 fibrinogen C-terminal domain.
  • ドメイン
    The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 別名
    • AGPT2
    • ANG 2
    • ANG-2
    • ANG2
    • Angiopoietin 2a
    • Angiopoietin 2B
    • Angiopoietin-2
    • Angiopoietin2
    • ANGP2_HUMAN
    • ANGPT 2
    • Angpt2
    • Tie2 ligand
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab99971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab99971.

プロトコール

参考文献

This product has been referenced in:
  • Zhou L  et al. Plasma angiopoietin-2 is persistently elevated after non-small cell lung cancer surgery and stimulates angiogenesis in vitro. Medicine (Baltimore) 95:e4493 (2016). Human . Read more (PubMed: 27512865) »
  • Genre F  et al. Antitumour necrosis factor-a therapy modulates angiopoietin-2 serum levels in non-diabetic ankylosing spondylitis patients. Ann Rheum Dis 72:1265-7 (2013). Read more (PubMed: 23482472) »

See all 2 Publications for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab99971.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録