Human Angiogenesis 抗体 Array - Membrane (23 targets) (ab169808)

製品の概要

  • 製品名
    Human Angiogenesis Antibody Array - Membrane (23 targets)
    Angiogenesis Antibody Array antibody arrays 製品一覧
  • サンプルの種類
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • アッセイタイプ
    Semi-quantitative
  • 種交差性
    交差種: Human
  • 製品の概要

    ab169808 is for simultaneous detection of 23 Human Angiogenic Factors. Suitable for all liquid sample types.


    Targets: Angiopoietin 1, Angiopoietin 2, Angiostatin, Endostatin, G-CSF, GM-CSF, I-309, IL-10, IL-1 alpha, IL-1 beta, IL-2, IL-4, I-TAC, MCP-3, MCP-4, MMP-1, MMP-9, PECAM-1, Tie-2, TNF alpha, uPAR, VEGFR2, VEGFR3


    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


    Learn more about membrane antibody arrays

  • 特記事項

    A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

  • アプリケーション
    適用あり: Multiplex Protein Detectionmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab169808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Multiplex Protein Detection Use at an assay dependent concentration.

画像

  • Typical results obtained with Abcam Membrane Antibody Arrays. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

プロトコール

参考文献

ab169808 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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