Human Amphiregulin ELISA Kit (AREG) (ab99975)


  • 製品名
    Human Amphiregulin ELISA Kit (AREG)
    Amphiregulin キット 製品一覧
  • 検出方法
  • サンプルの種類
    Cell culture supernatant, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 10 pg/ml
  • 検出範囲
    16.46 pg/ml - 4000 pg/ml
  • 添加回収試験

    83 %

    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 100.4 89% - 111%
    Plasma 72.7 64% - 87%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Amphiregulin (AREG) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Amphiregulin in plasma, and cell culture supernatants. (Human Amphiregulin concentration is pretty low in normal plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human Amphiregulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Amphiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Amphiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Amphiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    We have not been able to detect endogenous Human Amphiregulin in normal serum with ab99975, only in serum spiked with Human Amphiregulin.

  • 特記事項

    Optimisation may be required with urine samples.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム


  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    Amphiregulin Microplate (12 x 8 well strips) 1 unit
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human Amphiregulin (lyophilized) 2 vials
    Recombinant Human Amphiregulin Standard 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究分野
  • 関連性
    The mature secreted form of amphiregulin is an 84-amino acid residue glycosylated polypeptide which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR; these are EGF, TGFa, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. Amphiregulin expression is induced by phorbol ester, estrogen, androgen, and other EGFR ligands. In vitro, amphiregulin functions as an autocrine growth stimulator to drive proliferation of colon carcinoma cells, normal and oncogene-transformed mammary epithelial cells, cervical carcinoma cells, prostate cancer cells, and keratinocytes. Amphiregulin is important in the development of human placenta and murine mammary gland. It is reportedly overexpressed in human cancers of breast, colon, stomach and pancreas.
  • 細胞内局在
    Cell Membrane
  • 別名
    • A REG
    • AR
    • AREG
    • Colorectum cell derived growth factor
    • CRDGF
    • MGC13647
    • OTTHUMP00000219184
    • Schwannoma derived growth factor
    • SDGF
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab99975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab99975.

  • Representative Standard Curve using ab99975.



ab99975 has not yet been referenced specifically in any publications.

レビューと Q&A

A lot of our kits are very accommodating to many different samples types so lysates will likely work. If you decide to tests this kit with lysate samples, we recommend diluting the samples at least 5-fold with Assay Diluent B to minimize any...

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