Human alpha Fetoprotein ELISA Kit (AFP) (ab108838)

製品の概要

  • 製品名Human alpha Fetoprotein ELISA Kit (AFP)
    alpha 1 Fetoprotein キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.8%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.1%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 0.15 ng/ml
  • 検出範囲
    0.156 ng/ml - 10 ng/ml
  • 添加回収試験

    98 %

  • 全工程の試験時間
    4h 0m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s alpha Fetoprotein (AFP) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha Fetoprotein levels in plasma, serum and cell culture supernatants.

    An alpha Fetoprotein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha Fetoprotein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha Fetoprotein captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • 組織特異性Plasma. Synthesized by the fetal liver and yolk sac.
  • 配列類似性Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 発生段階Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • 翻訳後修飾Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Afp
    • Alpha fetoglobulin
    • Alpha fetoprotein
    • Alpha fetoprotein precursor
    • Alpha-1-fetoprotein
    • Alpha-fetoglobulin
    • Alpha-fetoprotein
    • alpha-fetoprotein, Hereditary persistence of, included
    • FETA
    • FETA_HUMAN
    • Hereditary persistence of alpha fetoprotein
    • HPAFP
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human alpha Fetoprotein ELISA Kit (AFP) 画像

  • Representative Standard Curve using ab108838.

プロトコール

Human alpha Fetoprotein ELISA Kit (AFP) (ab108838) 使用論文

ab108838 has not yet been referenced specifically in any publications.

Product Wall

The solubility is very good. It can go to higher than 10 ug/ml. If you do in the ng/ml range, it will be in the >500 ng/ml

Let me know if there is anything else I can help you with.

Thanks no problem. If you have any further questions relating to our products please do not hesitate to contact us again.

Thank you for contacting us.

The alpha Fetoprotein Human ELISA Kit (ab108838) has not had FDA approval but this is not necessary if the kit is to be used for research purposes only. All of our products are not intended to be used for diagnost...

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Thank you for contacting us. I can confirm that all the mentioned kits (ab99992, ab108631, ab108635, ab108838) come with a 96-well strip plate (12x8).  Each strip (12 total) has 8 wells so the customer can use a strip at a time if they wish. I ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"