製品の概要

  • 製品名Human alpha Fetoprotein ELISA Kit
    alpha 1 Fetoprotein キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Control Sera 48 <=6.4%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Control Sera 48 <=6.5%
  • テスト
    1 x 96 test
  • サンプルの種類
    Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    0.35 ng/ml
  • 検出範囲
    5 ng/ml - 200 ng/ml
  • 添加回収試験

    98.33 %

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s alpha Fetoprotein Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of alpha Fetoprotein in serum and plasma.

     

    A 96-well plate has been precoated with anti-alpha Fetoprotein IgG antibodies. Samples, standards and the alpha Fetoprotein-HRP conjugate are added to the wells, where alpha Fetoprotein in the sample and standards binds to the precoated antibody and added alpha Fetoprotein-HRP conjugate binds to this antibody-AFP complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of alpha Fetoprotein in the sample and the intensity is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • 組織特異性Plasma. Synthesized by the fetal liver and yolk sac.
  • 配列類似性Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 発生段階Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • 翻訳後修飾Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Afp
    • Alpha fetoglobulin
    • Alpha fetoprotein
    • Alpha fetoprotein precursor
    • Alpha-1-fetoprotein
    • Alpha-fetoglobulin
    • Alpha-fetoprotein
    • alpha-fetoprotein, Hereditary persistence of, included
    • FETA
    • FETA_HUMAN
    • Hereditary persistence of alpha fetoprotein
    • HPAFP
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab108680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Human alpha Fetoprotein ELISA Kit 画像

  • Representative Standard Curve using ab108680

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Human alpha Fetoprotein ELISA Kit (ab108680) 使用論文

ab108680 has not yet been referenced specifically in any publications.

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