製品の概要

  • 製品名Human alpha 2 Macroglobulin ELISA Kit
    alpha 2 Macroglobulin キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.1%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma, Cell Lysate
  • アッセイタイプCompetitive
  • 検出感度
    = 1 µg/ml
  • 検出範囲
    1.25 µg/ml - 40 µg/ml
  • 添加回収試験

    96 %

  • 全工程の試験時間
    3h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s alpha 2 Macroglobulin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 2 Macroglobulin in plasma and serum.

    An alpha 2 Macroglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated alpha 2 Macroglobulin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of alpha 2 Macroglobulin captured in plate.

  • アプリケーション適用あり: Competitive ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • 組織特異性Secreted in plasma.
  • 配列類似性Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • 発生段階Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • A2m
    • A2MG_HUMAN
    • Alpha 2 M
    • Alpha 2M
    • Alpha-2-M
    • Alpha-2-macroglobulin
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
    • CPAMD5
    • DKFZp779B086
    • FWP007
    • S863 7
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent dilution.

Human alpha 2 Macroglobulin ELISA Kit 画像

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Alpha 2 Macroglobulin measured in biological fluids and cell culture supernatant showing quantity (ug) per mL of tested sample. Human serum diluted 400-1600 fold. Human plasma diluted 80-320 fold. Other samples undiluted.

プロトコール

Human alpha 2 Macroglobulin ELISA Kit (ab108888) 使用論文

This product has been referenced in:
  • Sunderic M  et al. Protein molecular forms of insulin-like growth factor binding protein-2 change with aging. Exp Gerontol 58C:154-158 (2014). Read more (PubMed: 25106097) »

See 1 Publication for this product

Product Wall

The lab has informed me that your sample collection method is fine and that the anti-coagulant will not affect the assay. You can include the anticoagulant.

Thank you for contacting us.

The lab said the other option besides sodium citrate 0.1M is the ready use tube that clinical lab used to draw
human plasma for your samples.


I hope this information is helpful to you. Pleas...

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Thank you for contacting us.

Unfortunately the laboratory has no data regarding on ATC samples, and cannot give a recommendation here.

Please do not hesitate to contact us if you need any more advice or information.

Thank you very much for your call yesterday and for your patience while I've been in touch with my colleagues regarding this standard reconstitution volume.

For this lot, the standard vial contains 160 ug of human alpha-2-macroglobulin. So, t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"