Human alpha 2 Macroglobulin ELISA Kit (ab108883)

製品の概要

  • 製品名Human alpha 2 Macroglobulin ELISA Kit
    alpha 2 Macroglobulin キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.4%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 8.1%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Saliva, Milk
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 2 ng/ml
  • 検出範囲
    0.002 µg/ml - 0.5 µg/ml
  • 添加回収試験

    98 %

  • 全工程の試験時間
    4h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s alpha 2 Macroglobulin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 2 Macroglobulin in saliva, milk, cerebrospinal fluid and cell culture supernatants.

    An alpha 2 Macroglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha 2 Macroglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha 2 Macroglobulin captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • 組織特異性Secreted in plasma.
  • 配列類似性Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • 発生段階Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • A2m
    • A2MG_HUMAN
    • Alpha 2 M
    • Alpha 2M
    • Alpha-2-M
    • Alpha-2-macroglobulin
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
    • CPAMD5
    • DKFZp779B086
    • FWP007
    • S863 7
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human alpha 2 Macroglobulin ELISA Kit 画像

  • Representative Standard Curve using ab108883.

Human alpha 2 Macroglobulin ELISA Kit (ab108883) 使用論文

This product has been referenced in:
  • Sturtzel C  et al. The transcription factor MEF2C negatively controls angiogenic sprouting of endothelial cells depending on oxygen. PLoS One 9:e101521 (2014). Read more (PubMed: 24988463) »

See 1 Publication for this product

Product Wall



Unfortunately, I can confirm that the antibodies used in these kits have been raised against recombinant protein and purified proteins. The exact epitope of the antibodies have not been investigated further. We are therefore not able to provi...

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Abreview for alpha 2 Macroglobulin Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Cell (HepG2)
Specification: HepG2
Amount of Sample: 0.125ug standard µg
Type of Kit Used: ELISA
Positive control: Provided alpha 2 Macroglobulin standard
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Recognized human A2M. Did not recognize bovine A2M in FBS.
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Abcam user community

Verified customer

投稿 Jul 03 2012

Thank you for your inquiry. ab108884 is an alpha 1 microglobulin human ELISA kit, Swiss Prot P02760. Alpha1-microglobulin (1M), also called protein HC, is a tubular plasma and tissue protein that belongs to the lipocalin transport protein superfami...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"