Human alpha 2 Macroglobulin ELISA Kit (ab108883)
Key features and details
- Sensitivity: 1.1 ng/ml
- Range: 7.8 ng/ml - 125 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Saliva
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human alpha 2 Macroglobulin ELISA Kit
alpha 2 Macroglobulin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 5.5% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 10.3% -
サンプルの種類
Cell culture supernatant, Saliva, Milk, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 1.1 ng/ml -
検出範囲
7.8 ng/ml - 125 ng/ml -
添加回収試験
97 %
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全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s alpha 2 Macroglobulin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 2 Macroglobulin in saliva, milk, cerebrospinal fluid and cell culture supernatants.
An alpha 2 Macroglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha 2 Macroglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha 2 Macroglobulin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated alpha 2 Macroglobulin Antibody 1 x 120µl alpha 2 Macroglobulin Microplate (12 x 8 well strips) 1 unit alpha 2 Macroglobulin Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. -
組織特異性
Secreted in plasma. -
配列類似性
Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. -
発生段階
Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation. -
細胞内局在
Secreted. - Information by UniProt
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別名
- A2m
- A2MG_HUMAN
- Alpha 2 M
see all -
参照データベース
- Entrez Gene: 2 Human
- Omim: 103950 Human
- SwissProt: P01023 Human
- Unigene: 212838 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab108883 は 3 報の論文で使用されています。
- Wang C et al. Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans. Front Endocrinol (Lausanne) 12:830691 (2021). PubMed: 35185790
- Whiten DR et al. Single-Molecule Characterization of the Interactions between Extracellular Chaperones and Toxic a-Synuclein Oligomers. Cell Rep 23:3492-3500 (2018). PubMed: 29924993
- Sturtzel C et al. The transcription factor MEF2C negatively controls angiogenic sprouting of endothelial cells depending on oxygen. PLoS One 9:e101521 (2014). PubMed: 24988463