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Abcam’s alpha 1 Microglobulin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 1 Microglobulin concentrations in Human cell culture supernatants, serum, plasma, urine, saliva, CSF and milk.
An alpha 1 Microglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha 1 Microglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha 1 Microglobulin captured in plate.
|内容||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent M Concentrate||1 x 30ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|50X Biotinylated Human alpha 1 Microglobulin Antibody||1 x 120µl|
|alpha 1 Microglobulin Microplate (12 x 8 well strips)||1 unit|
|alpha 1 Microglobulin Standard||1 vial|
|Chromogen Substrate||1 x 8ml|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab108884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
Alpha 1 Microglobulin measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample. Human serum and plasma samples diluted 10000-40000 fold. Human urine and milk diluted 100-800 fold. Human saliva diluted 8-16 fold. HepG2 supernatant diluted 1000 fold. MG-63 supernatant tested undiluted - below detection. Mouse and rat samples diluted 10 fold - below detection.