Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab108798)


  • 製品名Human alpha 1 Antitrypsin ELISA Kit (SERPINA1)
    alpha 1 Antitrypsin キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    サンプル N 平均値 SD CV%
    Overall 3.7%
    サンプル N 平均値 SD CV%
    Overall 8.6%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Saliva, Milk, Serum, Plasma, Cell Lysate
  • アッセイタイプCompetitive
  • 検出感度
    = 0.018 µg/ml
  • 検出範囲
    0.039 µg/ml - 40 µg/ml
  • 添加回収試験

    98 %

  • 全工程の試験時間
    3h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s alpha 1 Antitrypsin (SERPINA1) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 1 antitrypsin levels in plasma and serum.

    An alpha 1 antitrypsin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated alpha 1 Antitrypsin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of alpha 1 antitrypsin captured in plate.

  • アプリケーション適用あり: Competitive ELISAmore details
  • 試験プラットフォームMicroplate


  • 機能Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
  • 組織特異性Ubiquitous. Expressed in leukocytes and plasma.
  • 関連疾患Alpha-1-antitrypsin deficiency
  • 配列類似性Belongs to the serpin family.
  • ドメインThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • 翻訳後修飾N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
  • 細胞内局在Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 別名
    • A1A
    • A1AT
    • A1AT_HUMAN
    • AAT
    • Alpha 1 antiproteinase
    • Alpha 1 antitrypsin
    • Alpha 1 antitrypsin null
    • Alpha 1 protease inhibitor
    • Alpha-1 protease inhibitor
    • Alpha-1-antiproteinase
    • alpha1 proteinase inhibitor
    • Alpha1AT
    • Dom1
    • PI
    • PI1
    • PRO2275
    • Serine (or cysteine) proteinase inhibitor clade A member 1
    • Serine protease inhibitor 1-1
    • Serine protease inhibitor A1a
    • Serpin A1
    • Serpin A1a
    • Serpin peptidase inhibitor clade A member 1
    • Serpina1
    • Short peptide from AAT
    • SPAAT
    • Spi1-1
    see all
  • 参照データベース


Our Abpromise guarantee covers the use of ab108798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent concentration.

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) 画像

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Alpha 1 Antitrypsin measured in biological fluids showing quantity (μg) per mL of tested sample. Serum and plasma samples diluted 100-8100 fold. Human milk and saliva diluted 10 fold. Hu urine diluted 1000 fold.


Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab108798) 使用論文

ab108798 has not yet been referenced specifically in any publications.

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ab108798 is for plasma and serum samples.

ab108799 is for urine, milk, saliva and cell culture samples

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You can use ab108799 for serum, you will just have to optimize the dilutions to get on the standard curve. Our serum samples were at 6000X.

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We unfortunately have not tested these products with sputum samples. Theoretically, these products will be OK however we cannot guarantee as we do not have tested data. The best way for using these products is by i...

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