製品の概要

  • 製品名Human Alpha 1 acid Glycoprotein ELISA Kit (AGP)
    Alpha 1 Acid Glycoprotein キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 9%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Saliva, Milk, Urine
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 3 ng/ml
  • 検出範囲
    3.125 ng/ml - 200 ng/ml
  • 添加回収試験

    = 97 %

  • 全工程の試験時間
    4h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Alpha 1 acid Glycoprotein (AGP) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of AGP levels in cell culture supernatant, saliva, milk, and urine.

    An Alpha 1 acid Glycoprotein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Alpha 1 acid Glycoprotein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Alpha 1 acid Glycoprotein captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Appears to function in modulating the activity of the immune system during the acute-phase reaction.
  • 組織特異性Expressed by the liver and secreted in plasma.
  • 配列類似性Belongs to the calycin superfamily. Lipocalin family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • A1AG1_HUMAN
    • AGP
    • AGP 1
    • AGP A
    • AGP1
    • Alpha 1 acid glycoprotein
    • Alpha-1-acid glycoprotein 1
    • Epididymis secretory sperm binding protein Li 153w
    • HEL S 153w
    • OMD 1
    • ORM
    • ORM1
    • Orosomucoid 1
    • Orosomucoid-1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Human Alpha 1 acid Glycoprotein ELISA Kit (AGP) 画像

  • Representative Standard Curve using ab108852.

プロトコール

Human Alpha 1 acid Glycoprotein ELISA Kit (AGP) (ab108852) 使用論文

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Thank you for your inquiry.

I can recommend ab108854 for your customer since this kit is tested and guaranteed for plasma and serum samples. ab108852 is not.

Please find the protocol booklet for ab108854 attached.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"