製品の概要

  • 製品名Human Albumin ELISA Kit
    Albumin キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 3.8%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 8.6%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプCompetitive
  • 検出感度
    = 1.5 µg/ml
  • 検出範囲
    1.563 µg/ml - 100 µg/ml
  • 添加回収試験

    97 %

  • 全工程の試験時間
    2h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Albumin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the measurement of albumin in Human plasma and serum.


    An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated albumin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of albumin captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human Serum Albumin ELISA Kit (ab179887) from our SimpleStep ELISA® range.

  • アプリケーション適用あり: Competitive ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • 組織特異性Plasma.
  • 関連疾患Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • 配列類似性Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 翻訳後修飾Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • alb
    • ALBU_HUMAN
    • Albumin (32 AA)
    • Albumin (AA 34)
    • cell growth inhibiting protein 42
    • DKFZp779N1935
    • GIG20
    • GIG42
    • growth-inhibiting protein 20
    • OTTHUMP00000220436
    • OTTHUMP00000220438
    • OTTHUMP00000220439
    • PRO0883
    • PRO0903
    • PRO1341
    • PRO1708
    • PRO2044
    • PRO2619
    • PRO2675
    • Serum albumin
    • UNQ696/PRO1341
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab108787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent concentration.

Human Albumin ELISA Kit 画像

  • Representative Standard Curve using ab108787.

プロトコール

Human Albumin ELISA Kit (ab108787) 使用論文

ab108787 has not yet been referenced specifically in any publications.

Product Wall

We have used human plasma at a dilution of 10,000x. Rat plasma was also tested at 50x but there was no cross-reactivity.

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining consistent results from this kit.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forELISA and h...

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Thank you very much for your call last week and for your patience while I have worked on your enquiry.

The lab has senta vial of the wash buffer, which we are sending to you free of charge on the order ***. This vial should arrive tomorrow. <...

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Thank you very much for your call this week and for your patience while I've been in touch with the lab.

The lab scientists do not recommend using a different buffer with this kit, as the results will be less than optimal. I'm arranging for a...

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DISCOUNT CODE: XXXXXXX
Expiration date: 15 June 2012

I am very pleased to hear you would like to accept our offer and test ab108787 with cell culture supernatant. This code will give you 1 free Kit before the expiration date. To redeem ...

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Thank you for your email. Dr Tanya is out of office this week so I am dealing with her inquiries.
I am happy in issuing a discount code. Could you send the email and contact address of end user?
Cheers!

Hope you are well?
Thank you for your enquiry and interest.
We would be very much interested in these data. Will the customer measure the secreted Albumin into the medium? Would it be possible to get some further information about the experim...

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Thank you for your inquiry and you positive feedback.

Please see my answers to your questions below:

1. I can confirm that the kits ab108902 (Transferrin Human ELISA Kit) and ab108788 (Albumin Human ELISA kit) were especially opti...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"