Human Adiponectin ELISA Kit (ab99968)
Key features and details
- Sensitivity: 25 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
-
製品名
Human Adiponectin ELISA Kit
Adiponectin キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 25 pg/ml -
添加回収試験
< 96 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 96.59 83% - 107% Serum 93.57 83% - 104% Plasma 90.29 80% - 103% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Human Adiponectin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Adiponectin in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Adiponectin coated on a 96-well plate. Standards and samples are pipetted into the wells and Adiponectin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Adiponectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Adiponectin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
特記事項
Optimisation may be required with urine samples.
-
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 80x HRP-Streptavidin Concentrate 1 x 200µl Adiponectin Microplate with anti-human Adiponectin. 1 unit Assay Diluent A 2 x 30ml Assay Diluent C 2 x 15ml Biotinylated Anti-Human Adiponectin 2 vials Standard Recombinant Human Adiponectin (Lyophilized). 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
-
機能
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. -
組織特異性
Synthesized exclusively by adipocytes and secreted into plasma. -
関連疾患
Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance. -
配列類似性
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
ドメイン
The C1q domain is commonly called the globular domain. -
翻訳後修飾
Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation. -
細胞内局在
Secreted. - Information by UniProt
-
別名
- 30 kDa adipocyte complement related protein
- 30 kDa adipocyte complement-related protein
- ACDC
see all -
参照データベース
- Entrez Gene: 9370 Human
- Omim: 605441 Human
- SwissProt: Q15848 Human
- Unigene: 80485 Human
画像
-
Adiponectin measured in biological fluids showing quantity (pg) per mL of tested sample. Human serum and plasma were diluted 2700-24300 fold. Rat and mouse serum were diluted 1-3 fold. Human urine and saliva were diluted 300-2700 fold.
-
Representative Standard Curve using ab99968
-
Representative Standard Curve using ab99968
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (23)
ab99968 は 23 報の論文で使用されています。
- Mackowiak-Lewandowicz K et al. Chronic kidney disease predictors in obese adolescents. Pediatr Nephrol 37:2479-2488 (2022). PubMed: 35211791
- He Z et al. Effects of Oral Vitamin C Supplementation on Liver Health and Associated Parameters in Patients With Non-Alcoholic Fatty Liver Disease: A Randomized Clinical Trial. Front Nutr 8:745609 (2021). PubMed: 34595203
- Lorè NI et al. CXCL10 levels at hospital admission predict COVID-19 outcome: hierarchical assessment of 53 putative inflammatory biomarkers in an observational study. Mol Med 27:129 (2021). PubMed: 34663207
- Metwalli O et al. Study of Some Inflammatory Mediators in the Serum of Patients With Atherosclerosis and Acute Myocardial Infarction. Cureus 13:e18450 (2021). PubMed: 34745775
- Martínez-Soto JM et al. Increased serum ferritin is associated with oxidized low-density lipoprotein in prediabetes patients: A pilot study. Heliyon 7:e06720 (2021). PubMed: 33912708