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Our Abpromise guarantee covers the use of ab78467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).|
ab78467 staining zebrafish eye sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 10%BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 21°C, followed by staining with ab78467 at 1/100 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. HuC expression can be observed in amacrine and retinal ganglion cells (in green).
ab78467 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"