製品の概要

  • 製品名Anti-HuC antibody
    HuC 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HuC
  • アプリケーション適用あり: WB, IHC-Frmore details
  • 種交差性
    交差種: Rat, Human, Zebrafish
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide near the N terminus of human/rat/mouse HuC

  • ポジティブ・コントロール
    • Adult Rat brain and Rat spinal cord tissue lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab78467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-Fr 1/100.

ターゲット情報

  • 機能Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF mRNA. May also bind poly-A tracts via RRM 3 (By similarity). May be involved in neuronal differentiation and maintenance.
  • 組織特異性Brain specific.
  • 配列類似性Belongs to the RRM elav family.
    Contains 3 RRM (RNA recognition motif) domains.
  • ドメインRRM 1 and RRM 2 bind cooperatively to AU-rich sequences in target mRNAs. RRM 3 binds to poly-A mRNA sequences.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ELAV (embryonic lethal abnormal vision Drosophila) like 3 (Hu antigen C) antibody
    • ELAV like protein 3 antibody
    • ELAV-like protein 3 antibody
    • ELAV3_HUMAN antibody
    • Elavl3 antibody
    • Embryonic lethal, abnormal vision, drosophila, homolog-like antibody
    • Hu antigen C antibody
    • Hu-antigen C antibody
    • HuC antibody
    • HUCL antibody
    • MGC20653 antibody
    • Paraneoplastic cerebellar degeneration associated antigen antibody
    • Paraneoplastic cerebellar degeneration-associated antigen antibody
    • Paraneoplastic limbic encephalitis antigen 21 antibody
    • PLE21 antibody
    see all

Anti-HuC antibody 画像

  • ab78467 staining zebrafish eye sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 10%BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 21°C, followed by staining with ab78467 at 1/100 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. HuC expression can be observed in amacrine and retinal ganglion cells (in green).

    See Abreview

  • Anti-HuC antibody (ab78467) at 0.5 µg/ml + rat brain tissue lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP

    Predicted band size : 40 kDa
    Observed band size : 40 kDa

Anti-HuC antibody (ab78467) 使用論文

This product has been referenced in:
  • Arslan-Ergul A  et al. Short-term dietary restriction in old zebrafish changes cell senescence mechanisms. Neuroscience 334:64-75 (2016). IHC ; Zebrafish . Read more (PubMed: 27475251) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Colon
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 23 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Brain
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 23 2015

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Sample Zebrafish Tissue sections (Retina, Amacrine cell, Retinal Ganglion Cell)
Specification Retina, Amacrine cell, Retinal Ganglion Cell
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Ryan MacDonald

Verified customer

投稿 May 28 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"