製品の概要

  • 製品名Anti-Hub1 antibody
  • 製品の詳細
    Rabbit polyclonal to Hub1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Saccharomyces cerevisiae
  • 免疫原

    Recombinant full length protein (S. cerevisiae).

  • ポジティブ・コントロール
    • Most yeast cell lysates can be used as a positive control without induction or stimulation.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab4754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 9.7 kDa.
ELISA 1/1000 - 1/5000.

ターゲット情報

  • 関連性Hub1 may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog.
  • 参照データベース
    • 別名
      • Hub1 protein antibody
      • HUB1 target protein 1 antibody
      • Hub1p antibody
      • Ubiquitin like protein modifier antibody

    Anti-Hub1 antibody 画像

    • ab4754 was tested by immunoblot against yeast lysates expressing the Hub1-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1/1000 dilution of Goat polyclonal to GFP (ab6673) followed by reaction with a 1/15000 dilution of Donkey polyclonal to Goat IgG (HRP)(ab7125). Panel C shows specific reaction with Hub1 using a 1/500 dilution of ab4754 followed by reaction with a 1/15000 dilution of Goat polyclonal to Rabbit IgG (HRP) (ab7090). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-Hub1 is highly specific and does not cross react with other UBLs. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M. Malakhov, www.lifesensors.com, personal communication.

    Anti-Hub1 antibody (ab4754) 使用論文

    ab4754 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"