製品の概要

  • 製品名Anti-htrA1 antibody
    htrA1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to htrA1
  • 特異性Targets N terminal of htrA1.
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human htrA1 aa 116-147 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q92743
    (Peptide available as ab112516)

  • ポジティブ・コントロール
    • WB: Mouse brain tissue lysate. IHC-P: Human kidney tissue. Flow Cyt: HeLa and HepG2 cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度Ammonium Sulphate Precipitation
  • 特記事項(精製)This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab38611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).Can be blocked with Human htrA1 peptide (ab112516).
IHC-P 1/10 - 1/50.

ターゲット情報

  • 機能Protease that regulate the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Represses signaling by TGF-beta family members.
  • 組織特異性Expressed in a variety of tissues, with strongest expression in placenta.
  • 関連疾患Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7) [MIM:610149]. ARMD is the leading cause of vision loss and blindness among older individuals in the developed word. It is classified as either dry (nonneovascular) or wet (neovascular). ARMD7 is a wet form, in which new blood vessels form and break beneath the retina. This leakage causes permanent damage to surrounding retinal tissue, distorting and destroying central vision. Wet ARMD is more prevalent among Asians than Caucasians.
    Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL) [MIM:600142]. CARASIL is characterized by nonhypertensive cerebral small-vessel arteriopathy with subcortical infarcts, alopecia, and spondylosis, with an onset in early adulthood. On neuropathological examination, arteriosclerosis associated with intimal thickening and dense collagen fibers, loss of vascular smooth-muscle cells, and hyaline degeneration of the tunica media has been observed in cerebral small arteries.
  • 配列類似性Belongs to the peptidase S1B family.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 Kazal-like domain.
    Contains 1 PDZ (DHR) domain.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARMD7 antibody
    • CARASIL antibody
    • High-temperature requirement A serine peptidase 1 antibody
    • HtrA antibody
    • HtrA serine peptidase 1 antibody
    • HTRA1 antibody
    • HTRA1_HUMAN antibody
    • IGFBP5 protease antibody
    • L56 antibody
    • ORF480 antibody
    • Protease serine 11 (IGF binding) antibody
    • protease serine 11 antibody
    • PRSS11 antibody
    • Serine protease 11 antibody
    • Serine protease HTRA1 antibody
    • Serine protease HTRA1 precursor antibody
    see all

Anti-htrA1 antibody 画像

  • Anti-htrA1 antibody (ab38611) at 1/2000 dilution + Mouse brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 51 kDa

    Blocking buffer 5% NFDM/TBST

    Diluting buffer 5% NFDM/TBST

  • Anti-htrA1 antibody (ab38611) + Mouse brain tissue lysate.

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
    Additional bands at : 28 kDa,35 kDa. We are unsure as to the identity of these extra bands.
  • ab38611 staining htrA1 in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in 10mM sodium citrate + 0.05% Tween. Samples were incubated with primary antibody (1/50 in PBS) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling htrA1 with ab38611. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling htrA1 (green) with ab38611. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. Nuclei were stained with DAPI (blue).

  • ICC/IF image of ab38611 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38611, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-htrA1 antibody (ab38611) 使用論文

This product has been referenced in:
  • Searcy JL  et al. Impact of age on the cerebrovascular proteomes of wild-type and Tg-SwDI mice. PLoS One 9:e89970 (2014). IHC ; Mouse . Read more (PubMed: 24587158) »
  • Nakayama M  et al. Overexpression of HtrA1 and exposure to mainstream cigarette smoke leads to choroidal neovascularization and subretinal deposits in aged mice. Invest Ophthalmol Vis Sci 55:6514-23 (2014). WB . Read more (PubMed: 25205867) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample Human Tissue sections (human pancreas carcinoma)
Specification human pancreas carcinoma
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Mrs. Maria Cecilia Ybanez

Verified customer

投稿 Mar 17 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate, 0.05% Tween
Sample Human Tissue sections (human liver)
Specification human liver
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

投稿 Dec 24 2013

This product has been tested under reducing and denaturing conditions.

Thank you for your reply. Blocking longer may help to further reduce the additional background bands observed, however as shown in the image on our datasheet, this antibody is known to detect some non-specific bands in WB. Could you p...

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Thank you for contacting us. I am sorry that your customer is obtaining high background using ab38611 in WB. What species did the samples come from and what tissue type or cell line was used? Which lanes correspond to the cytoplasmic and nucle...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"