製品の概要

  • 製品名
    Anti-htrA antibody
  • 製品の詳細
    Rabbit polyclonal to htrA
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    Reacts with Synechocystis sp. PCC 6803. Possibly crossreacts with Anabaena sp. PCC 7120.
  • 免疫原

    Synthetic peptide:

    QMPPMPNERVQRGT

    , corresponding to amino acids 159-172 of Synechocystis sp. PCC 6803 htrA

  • ポジティブ・コントロール
    • Synechocystis sp. PCC 6803 membrane proteins.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: Whole serum
  • 精製度
    Whole antiserum
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab65481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 48 kDa).

ターゲット情報

  • 関連性
    htrA belongs to a serine type Deg peptidase family, widespread among bacteria and eukaryotes. This family includes three related members htrA (DegP), HhoA (DegQ) and HhoB (DegS). htrA is a highly hydrophilic protein and probably resides in a soluble compartment. The functions of htrA in cyanobacteria are not studied yet, however, in E. coli, htrA is a heat stress protein that functions in a homohexameric state in the periplasm.
  • 細胞内局在
    Periplasm
  • 別名
    • htrA antibody
    • Serine protease antibody
    • Slr1204 antibody

画像

  • Anti-htrA antibody (ab65481) at 1/2000 dilution + Synechocystis sp. PCC 6803 membrane proteins at 3 µg

    Secondary
    anti-rabbit at 1/30000 dilution
    Developed using the ECL technique

    Predicted band size : 48 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab65481 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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