The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 48 kDa).
htrA belongs to a serine type Deg peptidase family, widespread among bacteria and eukaryotes. This family includes three related members htrA
(DegP), HhoA (DegQ) and HhoB (DegS). htrA is a highly hydrophilic protein and probably resides in a soluble compartment. The functions of htrA in
cyanobacteria are not studied yet, however, in E. coli, htrA is a heat stress protein that functions in a homohexameric state in the periplasm.
Serine protease antibody
Western blot - htrA antibody (ab65481)
Anti-htrA antibody (ab65481) at 1/2000 dilution + Synechocystis sp. PCC 6803
membrane proteins at 3 µg
Secondary anti-rabbit at 1/30000 dilution Developed using the ECL technique