Anti-CENH3 抗体 (ab72001)
Key features and details
- Rabbit polyclonal to CENH3
- Suitable for: ICC, WB
- Reacts with: Arabidopsis thaliana
- Isotype: IgG
製品の概要
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製品名
Anti-CENH3 antibody -
製品の詳細
Rabbit polyclonal to CENH3 -
由来種
Rabbit -
アプリケーション
適用あり: ICC, WBmore details -
種交差性
交差種: Arabidopsis thaliana -
免疫原
Synthetic peptide corresponding to Arabidopsis thaliana CENH3 (N terminal).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72001の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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特記事項 |
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ICC
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ターゲット情報
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関連性
HTR12 is a histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. It is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation and may serve as an epigenetic mark that propagates centromere identity through replication and cell division. -
細胞内局在
Nucleus. Centromere. Kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres. -
参照データベース
- Entrez Gene: 839104 Arabidopsis thaliana
- SwissProt: Q8RVQ9 Arabidopsis thaliana
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別名
- CENH3 antibody
- Centromeric histone CENH3 antibody
- F6F3.17 antibody
see all
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (6)
ab72001 は 6 報の論文で使用されています。
- Capitao C et al. A CENH3 mutation promotes meiotic exit and restores fertility in SMG7-deficient Arabidopsis. PLoS Genet 17:e1009779 (2021). PubMed: 34591845
- Naish M et al. The genetic and epigenetic landscape of the Arabidopsis centromeres. Science 374:eabi7489 (2021). PubMed: 34762468
- Xu RY et al. The Arabidopsis anaphase-promoting complex/cyclosome subunit 8 is required for male meiosis. New Phytol 224:229-241 (2019). PubMed: 31230348
- Antosch M et al. Mitotic lifecycle of chromosomal 3xHMG-box proteins and the role of their N-terminal domain in the association with rDNA loci and proteolysis. New Phytol 208:1067-77 (2015). PubMed: 26213803
- Karimi-Ashtiyani R et al. Point mutation impairs centromeric CENH3 loading and induces haploid plants. Proc Natl Acad Sci U S A 112:11211-6 (2015). WB . PubMed: 26294252
- Mérai Z et al. The AAA-ATPase molecular chaperone Cdc48/p97 disassembles sumoylated centromeres, decondenses heterochromatin, and activates ribosomal RNA genes. Proc Natl Acad Sci U S A 111:16166-71 (2014). PubMed: 25344531