製品の概要

  • 製品名Anti-HTLV I p24 antibody [46/3.24.4]
  • 製品の詳細
    Mouse monoclonal [46/3.24.4] to HTLV I p24
  • 特異性Reacts with Human T-Lymphotropic Virus Type I (HTLV I) core p24. Cross reactivity with p24 from HTLV II has been observed.
  • アプリケーション適用あり: ICC/IF, Radioimmunoprecipitation, WB, ELISAmore details
  • 免疫原

    HTVL viral lysate

  • 特記事項


    The antibody is used to detect HTLV I core protein in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. The antibody is also valuable for the affinity isolation of HTLV I core protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)Purified from serum-free culture supernatant.
  • 一次抗体 備考The antibody is used to detect HTLV I core protein in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. The antibody is also valuable for the affinity isolation of HTLV I core protein.
  • ポリ/モノモノクローナル
  • クローン名46/3.24.4
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution. PubMed: 19008946
Radioimmunoprecipitation Use at an assay dependent dilution.
WB Use a concentration of 1 - 10 µg/ml.
Antibody dilutions should be prepared using buffers containing suitable protein in order to stabilize antibody activity.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 関連性Human T-lymphotropic virus (HTLV) belongs to the family Retroviridae, genus Deltaretrovirus. It is a human, single-stranded RNA retrovirus that causes T-cell leukemia and T-cell lymphoma in adults and may also be involved in certain demyelinating diseases. Adult T-lymphotropic virus (ATLV) is a strain of this disease that effect primarily adults. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • 細胞内局在Core protein
  • 別名
    • CA antibody
    • Capsid protein p24 antibody
    • p24 antibody

Anti-HTLV I p24 antibody [46/3.24.4] 画像

  • Immunofluorescence analysis of HTLV-1-infected T cells, staining HTLV I p24 with ab9081.

    Cells were fixed with paraformaldehyde before incubating with 10% normal goat serum and 0.05% saponin for 30 minutes. Cells were incubated with primary antibody and a fluoroscein-conjugated anti-mouse IgG was used as the secondary antibody.
  • Immunofluorescence analysis of HeLa cells transiently transfected with pEYFP-N3-HTLV-1 Gag, staining HTLV I p24 with ab9081.

    Cells were fixed with paraformaldehyde, permeabilized with 0.05% Triton X-100 and blocked with 5% normal donkey serum for 30 minutes. Samples were incubated with primary antibody (1/150 in blocking solution) for 2 hours at room temperature. An AlexaFluor®488-conjugated donkey anti-mouse IgG (1/250) was used as the secondary antibody.

Anti-HTLV I p24 antibody [46/3.24.4] (ab9081) 使用論文

This product has been referenced in:
  • Martin-Latil S  et al. Transcytosis of HTLV-1 across a tight human epithelial barrier and infection of subepithelial dendritic cells. Blood 120:572-80 (2012). Read more (PubMed: 22589473) »
  • Grigsby IF  et al. Biophysical analysis of HTLV-1 particles reveals novel insights into particle morphology and Gag stochiometry. Retrovirology 7:75 (2010). WB, ICC/IF . Read more (PubMed: 20854688) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"