Reacts with HSV Type 1 (titer is 1:512 by Indirect IFA).
Not yet tested in other species.
HSV Type 2 Strain G (human).
Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
Purified IgG fraction covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is not present. Estimated molar HRP:IgG substitution is 2-3.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20 - 1/200. Dilution optimised using Chromogenic detection.
1/200 - 1/1000.
Use at an assay dependent dilution.
Herpes simplex type 2 (HSV2) belongs to a family that includes HSV1, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus. HSV1 and HSV2 are extremely difficult to distinguish from each other. These viruses have a DNA genome, an icosahedral protein coat and are encased in a lipid membrane derived from the nuclear membrane of the last host. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low. During the latent phase the viral DNA is integrated into the genome of the host cell.