製品の概要

  • 製品名
    Anti-HSV1 ICP0 antibody [5H7]
  • 製品の詳細
    Mouse monoclonal [5H7] to HSV1 ICP0
  • アプリケーション
    適用あり: WB, ELISA, ICC/IFmore details
  • 免疫原

    Herpes Virus

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Phosphate buffered saline
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    5H7
  • ミエローマ
    NS1/1-Ag4-1
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/8000.
ELISA 1/12800.
ICC/IF 1/25600.

ターゲット情報

  • 関連性
    Herpes simplex virus (HSV) encodes four nuclear phosphoproteins that contribute towards the full productive lytic cycle cascade in newly infected permissive cells. The IE175 (or ICP4) and IE63 (or ICP27) proteins have essential regulatory roles during the lytic cycle. A third, IE110 (or ICP0), appears to play only an accessory role in the presence of VP16, but in the absence of VP16 appears to be critical for initiation of reactivated infections.
  • 細胞内局在
    Nuclear
  • 別名
    • Alpha 0 protein antibody
    • Herpes siplex virus 1 antibody
    • HHV1 ICP0 antibody
    • HSV1 IE110 antibody
    • Human Herpesvirus 1 antibody
    • IE110 antibody
    • Immediate early nuclear phosphoprotein antibody
    • Immediate early protein IE110 antibody
    • Trans acting transcriptional protein ICP0 antibody
    • VMW110 antibody
    see all

Anti-HSV1 ICP0 antibody [5H7] 画像

  • ab6513 (1/25600) staining HSV1 ICP0 in human cells. Cells were PFA-fixed, permeabilized in 0.3% TritonX-100/PBS and blocked in 1% serum /30 minutes /25oC. A fluorescently labeled donkey anti-mouse antibody was used as the secondary.

    See Abreview

Anti-HSV1 ICP0 antibody [5H7] (ab6513) 使用論文

This product has been referenced in:
  • Wang Z  et al. NEAT1 modulates herpes simplex virus-1 replication by regulating viral gene transcription. Cell Mol Life Sci 74:1117-1131 (2017). WB . Read more (PubMed: 27783096) »
  • Millet R  et al. Impact of the MRN Complex on Adeno-Associated Virus Integration and Replication during Coinfection with Herpes Simplex Virus 1. J Virol 89:6824-34 (2015). Read more (PubMed: 25903339) »

See all 13 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HSV1 infected or transfected with plasmid)
Specification
HSV1 infected or transfected with plasmid
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX-100 in PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 12 2007

According to the datasheet, this antibody has not been tested for immunohistochemistry. I have looked through our catalogue and am very sorry to inform you that we currently do not stock products that suit to your requirement. If you cannot find...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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