Anti-HSV1 + HSV2 ICP5 Major Capsid Protein 抗体 [3B6] (ab6508)

製品の概要

  • 製品名
    Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6]
  • 製品の詳細
    Mouse monoclonal [3B6] to HSV1 + HSV2 ICP5 Major Capsid Protein
  • アプリケーション
    適用あり: WB, ELISA, ICC/IF, IHC-Pmore details
  • 免疫原

    Herpes Virus

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Phosphate buffered saline
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    3B6
  • ミエローマ
    NS1/1-Ag4-1
  • アイソタイプ
    IgG2b
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
ICC/IF
IHC-P
  • 追加情報
    ICC/IF: Use at an assay dependent dilution (PMID 18701599).
    IFA titer: HSV-1 1+ @ 1:3,200; HSV-2 1+ @ 1:3,200
    ELISA titer: HSV-1 0.100 @ >1:102,400; HSV-2 0.100 @ >1:102,400
    Western Blot Titer: HSV-1 >1:3,200; HSV-2 >1:3,200

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      Herpes simplex virus 1 and 2 (HSV-1 and HSV-2) are two species of the herpes virus family, Herpesviridae, which cause infections in humans. They are also called Human Herpes Virus 1 and 2 (HHV-1 and HHV-2) and are neurotropic and neuroinvasive viruses; they enter and hide in the human nervous system, accounting for their durability in the human body.
    • 別名
      • Capsid protein VP5 antibody
      • Herpes simplex virus 1 major capsid protein antibody
      • Herpes simplex virus 2 major capsid protein antibody
      • Human Herpes virus antibody
      • Major capsid protein antibody
      • MCP antibody
      • UL19 antibody
      • VP5 antibody
      see all

    Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6] 画像

    • ab6508 staining HSV1 + HSV2 ICP5 Major Capsid Protein in Human head and neck cancer tissue and Mouse xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 1 hour at 23°C; antigen retrieval was by heat mediation in a 10mM sodium citrate, pH6. Samples were incubated with primary antibody (1/100 in blocking buffer) for 1 hour at 4°C. A Biotin-conjugated anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.

      See Abreview

    • Immunofluorescence analysis of HSV infected cells, 7 hours (left) or 17 hours (right) post-infection, staining HSV1 + HSV2 ICP5 Major Capsid Protein with ab6508 at 1/200 dilution.

      Cells were fixed for 10 min in paraformaldehyde, permeabilized in 0.1% Triton X-100 and blocked in 4% goat serum, 1% bovine serum albumin, before incubating with primary antibody for 30 min at room temperature. An AlexaFluor®647-conjugated goat anti-mouse IgG1 was used as the secondary antibody.

    Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6] (ab6508) 使用論文

    This product has been referenced in:
    • Ott M  et al. Asna1/TRC40 that mediates membrane insertion of tail-anchored proteins is required for efficient release of Herpes simplex virus 1 virions. Virol J 13:175 (2016). Read more (PubMed: 27765046) »
    • Katzenell S & Leib DA Herpes Simplex Virus and Interferon Signaling Induce Novel Autophagic Clusters in Sensory Neurons. J Virol 90:4706-19 (2016). Read more (PubMed: 26912623) »

    See all 24 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Head and neck cancer, mouse xenograft)
    Specification
    Head and neck cancer, mouse xenograft
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate, pH=6
    Permeabilization
    No
    Blocking step
    Vector Lab Mouse Ig Blocking Reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
    Username

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    Verified customer

    投稿 May 14 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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