Anti-HSV1 + HSV2 ICP5 Major Capsid Protein 抗体 [3B6] (ab6508)

製品の概要

  • 製品名Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6]
  • 製品の詳細
    Mouse monoclonal [3B6] to HSV1 + HSV2 ICP5 Major Capsid Protein
  • アプリケーション適用あり: WB, ELISA, ICC/IF, IHC-Pmore details
  • 免疫原

    Herpes Virus

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPhosphate buffered saline
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名3B6
  • ミエローマNS1/1-Ag4-1
  • アイソタイプIgG2b
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
ICC/IF
IHC-P
  • 追加情報ICC/IF: Use at an assay dependent dilution (PMID 18701599).
    IFA titer: HSV-1 1+ @ 1:3,200; HSV-2 1+ @ 1:3,200
    ELISA titer: HSV-1 0.100 @ >1:102,400; HSV-2 0.100 @ >1:102,400
    Western Blot Titer: HSV-1 >1:3,200; HSV-2 >1:3,200

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性Herpes simplex virus 1 and 2 (HSV-1 and HSV-2) are two species of the herpes virus family, Herpesviridae, which cause infections in humans. They are also called Human Herpes Virus 1 and 2 (HHV-1 and HHV-2) and are neurotropic and neuroinvasive viruses; they enter and hide in the human nervous system, accounting for their durability in the human body.
    • 別名
      • Capsid protein VP5 antibody
      • Herpes simplex virus 1 major capsid protein antibody
      • Herpes simplex virus 2 major capsid protein antibody
      • Human Herpes virus antibody
      • Major capsid protein antibody
      • MCP antibody
      • UL19 antibody
      • VP5 antibody
      see all

    Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6] 画像

    • ab6508 staining HSV1 + HSV2 ICP5 Major Capsid Protein in Human head and neck cancer tissue and Mouse xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 1 hour at 23°C; antigen retrieval was by heat mediation in a 10mM sodium citrate, pH6. Samples were incubated with primary antibody (1/100 in blocking buffer) for 1 hour at 4°C. A Biotin-conjugated anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.

      See Abreview

    • Immunofluorescence analysis of HSV infected cells, 7 hours (left) or 17 hours (right) post-infection, staining HSV1 + HSV2 ICP5 Major Capsid Protein with ab6508 at 1/200 dilution.

      Cells were fixed for 10 min in paraformaldehyde, permeabilized in 0.1% Triton X-100 and blocked in 4% goat serum, 1% bovine serum albumin, before incubating with primary antibody for 30 min at room temperature. An AlexaFluor®647-conjugated goat anti-mouse IgG1 was used as the secondary antibody.

    Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6] (ab6508) 使用論文

    This product has been referenced in:
    • Lau SY & Crump CM HSV-1 gM and the gK/pUL20 Complex Are Important for the Localization of gD and gH/L to Viral Assembly Sites. Viruses 7:915-38 (2015). WB, ICC/IF ; Other . Read more (PubMed: 25746217) »
    • Zhong M  et al. Heat-shock protein 90 promotes nuclear transport of herpes simplex virus 1 capsid protein by interacting with acetylated tubulin. PLoS One 9:e99425 (2014). Read more (PubMed: 24901434) »

    See all 16 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Head and neck cancer, mouse xenograft)
    Specification Head and neck cancer, mouse xenograft
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate, pH=6
    Permeabilization No
    Blocking step Vector Lab Mouse Ig Blocking Reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
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    投稿 May 14 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"