製品の概要

  • 製品名Anti-HSV1 + HSV2 gB antibody [10B7]
    HSV1 + HSV2 gB 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [10B7] to HSV1 + HSV2 gB
  • アプリケーション適用あり: WB, ELISA, ICC/IFmore details
  • 免疫原

    Herpes Virus

  • ポジティブ・コントロール
    • African Green Monkey Kidney cultured cells(Vero), HSV-1 is strain MacIntyre, HSV-2 is strain G.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPhosphate buffered saline
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名10B7
  • ミエローマNS1/1-Ag4-1
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.

1/1600 (HSV2) - 1/12800 (HSV1).

ELISA 1/102400.
ICC/IF Use at an assay dependent concentration. 1/6400 (HSV1) - 1/25600 (HSV2).

ターゲット情報

  • 関連性There are two main types of Herpes Simplex Virus (HSV), 1 and 2. The primary difference between the two types is in where they typically establish latency in the body. HSV1 usually establishes latency in the trigeminal ganglion, a collection of nerve cells near the ear. From there, it tends to recur on the lower lip or face causing cold sores. HSV2 usually resides in the sacral ganglion at the base of the spine. From there, it recurs in the genital area but even this difference is not absolute as in rare cases it can infect any part of the body, including the eyes. Up to 30% of genital herpes are also caused by HSV1. Both HSV1 and 2 are highly contagious through contact.
  • 細胞内局在Virus envelope
  • 別名
    • Envelope glycoprotein B antibody
    • GB antibody
    • Glycoprotein B antibody
    • HSV1 antibody
    • HSV2 antibody
    • UL27 antibody
    see all

Anti-HSV1 + HSV2 gB antibody [10B7] 画像

  • Green: immunofluorescence staining of HSV1 or HSV2 of Vero (African green monkey) cells ( HSV-1 is strain MacIntyre, HSV-2 is strain G) on slides, using ab6506 (1:100) (secondary anti-mouse-FITC). Red: counterstain in the conjugate preparation.
  • Lane 1 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/12800 dilution
    Lane 2 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/1600 dilution

    Lane 1 : Vero cells infected with HSV1
    Lane 2 : Vero cells infected with HSV2


    Performed under reducing conditions.

Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) 使用論文

This product has been referenced in:
  • Bourgade K  et al. ß-Amyloid peptides display protective activity against the human Alzheimer's disease-associated herpes simplex virus-1. Biogerontology 16:85-98 (2015). Read more (PubMed: 25376108) »
  • Wang X  et al. Direct activation of RIP3/MLKL-dependent necrosis by herpes simplex virus 1 (HSV-1) protein ICP6 triggers host antiviral defense. Proc Natl Acad Sci U S A 111:15438-43 (2014). IHC ; Mouse . Read more (PubMed: 25316792) »

See all 5 Publications for this product

Product Wall

Thank you for contacting us.

The following article will be helpful for further reading; http://www.ncbi.nlm.nih.gov/pubmed/2475970

We can provide the blocking peptide , which was used for producing this antibody? Could you confirm if ...

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I am very pleased to hear you would like to accept our offer and test ab6506 in ICC on neurons. This code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for this ...

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Thank you very much for your interest in ab6506 and ab6509.

To our knowledge, these products have not been tested inneurons in culture. Therefore, I can offer a discount off a future purchase if you buy one or both of these products now, t...

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Thank you for your enquiry.

The immunogen used to generate the antibody clone [10B7] (ab6506) was the entire Herpes Virus. Unfortunately, the exact epitope recognised by this monoclonal antibody has not been mapped.

To our knowled...

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Thank you for confirming these details and for your cooperation.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued an alternative free of charge replacem...

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Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to ...

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Thank you for your inquiry. I heard back from the lab and unfortunately neither ab6506 nor ab33040 have been epitope mapped. We do not know if these antibodies will work in your sELISA since we do not know where the antibodies are binding. I am sorry t...

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Thank you for your enquiry. I have obtained some details on this antibody for you. As it is a virus neutralising antibody, it is highly likely that it binds to the extracellular domain. However, there is no information available on the precise epitope/...

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Thank you for your phone call. I apologize for the shortage that you have received and a replacement is being sent to you free of charge on order# 81816 which you should receive in approximately one week. I did contact the source of this product an...

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Hi Joel, Yes it does detect gB from both type 1 and type 2 HSV. Please see our testing data at: HSV gB Mab

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"