Anti-HSV1 gH 抗体 [BBH1] (ab110227)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [BBH1] to HSV1 gH
- Suitable for: IHC-P, ICC/IF, IP, WB
- Reacts with: Herpes simplex virus
製品の概要
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製品名
Anti-HSV1 gH antibody [BBH1]
HSV1 gH 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [BBH1] to HSV1 gH -
由来種
Mouse -
特異性
Cross-reactivity has not been checked and this antibody might cross with HSV-2 gH.
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アプリケーション
適用あり: IHC-P, ICC/IF, IP, WBmore details -
種交差性
交差種: Herpes simplex virus -
免疫原
Tissue, cells or virus corresponding to HSV1 gH. Herpes Simplex Virus Type 1.
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ポジティブ・コントロール
- ICC/IF: HEK-293T (human epithelial cell line from embryonic kidney) transfected with myc-tagged Herpes simplex virus 1 gH expression vector IP: HEK-293T (human epithelial cell line from embryonic kidney) transfected with myc-tagged Herpes simplex virus 1 gH expression vector WB: HEK-293T (human epithelial cell line from embryonic kidney) transfected with myc-tagged Herpes simplex virus 1 gH expression vector
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特記事項
This product has switched from a hybridoma to recombinant production method on 27th October 2023.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
BBH1 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110227の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/1000.
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ICC/IF |
1/200.
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IP |
1/50.
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WB |
1/1000.
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特記事項 |
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IHC-P
1/1000. |
ICC/IF
1/200. |
IP
1/50. |
WB
1/1000. |
ターゲット情報
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別名
- Glycoprotein H antibody
- Herpes Simplex Virus 1 glycoprotein H antibody
- HSV1 antibody
画像
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Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Herpes simplex virus 1 gH expression vector containing a myc-his tag. (B) HEK-293T cells transfected with empty vector containing a myc-his tag staning HSV1 gH with ab110227 at 1/1000 dilution (0.894 µg/ml), followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Positive staining on (A) HEK-293T transfected with a Herpes simplex virus 1 gH expression vector containing a myc-his tag. No staining on (B) HEK-293T cells transfected with empty vector containing a myc-his tag.
The section was incubated with ab110227 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue staning HSV1 gH with ab110227 at 1/1000 dilution (0.894 µg/ml), followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Negative control: no staining on human cerebrum.
The section was incubated with ab110227 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) transfected with myc-tagged Herpes simplex virus 1 gH expression vector cells labelling HSV1 gH with ab110227 at 1/200 (4.47 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic and membranous staining in 293T cells transfected with a Herpes simplex virus 1 gH expression vector containing a myc-His-tag.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Anti-Myc Rabbit polyclonal antibody (ab9106) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
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HSV1 gH was immunoprecipitated from 0.35 mg of 293T cells transfected with a Herpes simplex virus 1 gH expression vector containing a myc-His- tag whole cell lysate with ab110227 at 1/50 dilution (2μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab110227 at 1/1000 dilution. ab131366, VeriBlot for IP secondary antibody(HRP) was used as secondary antibody at 1/5000 dilution.
Lane 1(Input):293T cells transfected with a Herpes simplex virus 1 gH
expression vector containing a myc-His-tag whole cell lysate, 10 μg
Lane 2(+):293T cells transfected with a Herpes simplex virus 1 gH
expression vector containing a myc-His-tag whole cell lysate
Lane 3(-): Mouse IgG2a kppa monoclonal isotype control (ab18413) instead of ab110227 in 293T cells transfected with a Herpes simplex virus 1 gH expression vector containing a myc-His-tag whole cell lysateObserved MW(KDa):100
Exposure time:180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-HSV1 gH antibody [BBH1] (ab110227) at 1/1000 dilution
Lane 1 : 293T cells transfected with an empty vector containing a myc-His-tag whole cell lysate
Lane 2 : 293T cells transfected with a Herpes simplex virus 1 gH expression vector containing a myc-His-tag whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 48 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
ab213204 was used as a total protein control at 1/5000 dilution.
ab181602 was used as a GAPDH loading control at 1/200000 dilution.
Cross-reactivity has not been checked and this antibody might cross with HSV-2 gH.
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Western blot analysis of ab110227.
Each track was loaded with 2x10^5 cells infected with HSV1 strain SC16, or mock-infected cells.
BBH1 - ab110227, anti-gH monoclonal antibody (1mg/ml)
Shabba - anti-gH polyclonal antibody
LP14 - anti-gD polyclonal antibody
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab110227 は 5 報の論文で使用されています。
- Polpitiya Arachchige S et al. Analysis of herpes simplex type 1 gB, gD, and gH/gL on production of infectious HIV-1: HSV-1 gD restricts HIV-1 by exclusion of HIV-1 Env from maturing viral particles. Retrovirology 16:9 (2019). PubMed: 30940160
- Albecka A et al. HSV-1 Glycoproteins Are Delivered to Virus Assembly Sites Through Dynamin-Dependent Endocytosis. Traffic 17:21-39 (2016). PubMed: 26459807
- Lau SY & Crump CM HSV-1 gM and the gK/pUL20 Complex Are Important for the Localization of gD and gH/L to Viral Assembly Sites. Viruses 7:915-38 (2015). WB ; Other species . PubMed: 25746217
- Ren Y et al. Glycoprotein M is important for the efficient incorporation of glycoprotein H-L into herpes simplex virus type 1 particles. J Gen Virol 93:319-29 (2012). ICC/IF ; Other species . PubMed: 22012461
- Svobodova S et al. Analysis of the interaction between the essential herpes simplex virus 1 tegument proteins VP16 and VP1/2. J Virol 86:473-83 (2012). WB . PubMed: 22013045