製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to HSV1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC - Wholemount, IHC-P, IHC-FoFr, ICC/IF, ELISAmore details
  • 免疫原

    Tissue, cells or virus corresponding to HSV1. Whole rabbit corneal cells, infected with herpes simplex virus type I (strain Mac Intyre), were solubilized with detergent.

  • ポジティブ・コントロール
    • IHC-Wm: HSV1 in cornea from pVEGFA-GFP reporter mice at day 3 post-infection with HSV1 strain McKrae. IHC-P: HSV1-infected human tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • ミエローマ
    unknown
  • アイソタイプ
    IgG
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC - Wholemount Use at an assay dependent concentration. PubMed: 21998580
IHC-P Use at an assay dependent concentration.

This antibody may be diluted to a titer of 1:75-1:200 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature. Pretreat with citrate buffer pH 6.0.

IHC-FoFr Use at an assay dependent concentration. PubMed: 18042934
ICC/IF Use at an assay dependent concentration. PubMed: 27783096
ELISA Use at an assay dependent concentration. PubMed: 22003387

ターゲット情報

  • 関連性
    Herpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low.
  • 別名
    • Herpes simplex virus 1 antibody
    • Herpes simplex virus antibody
    • Herpes simplex virus type 1 antibody
    • HSV 1 antibody
    • Human herpesvirus 1 antibody
    • Human herpesvirus type 1 antibody
    see all

画像

  • Formalin-fixed, paraffin-embedded HSV1-infected human tissue stained for HSV1 with ab9533 in immunohistochemical analysis.

  • ab9533, staining HSV1 in cornea from pVEGFA-GFP reporter mice at day 3 post-infection with HSV1 strain McKrae.

    Corneas were fixed in 4% paraformaldehyde, and washed with PBS containing 1% Triton X-100 before being stained with primary antibody. A Dylight®549-conjugated donkey anti-rabbit IgG was used as the secondary antibody.

  • Formalin-fixed, paraffin-embedded HSV1-infected human tissue stained for HSV1 with ab9533 in immunohistochemical analysis.

参考文献

This product has been referenced in:
  • Wang Z  et al. NEAT1 modulates herpes simplex virus-1 replication by regulating viral gene transcription. Cell Mol Life Sci 74:1117-1131 (2017). IF . Read more (PubMed: 27783096) »
  • Agelidis AM  et al. Viral Activation of Heparanase Drives Pathogenesis of Herpes Simplex Virus-1. Cell Rep 20:439-450 (2017). Read more (PubMed: 28700944) »

See all 11 Publications for this product

レビューと Q&A

I am sorry but the specificity of the antibody has not been studies in detail, I cannot therefore share a great deal of information with you. The antibody has been raised against whole rabbit corneal cells, infected with herpes simplex virus type I. Th...

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lip)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6.0 + .05% Tween-20
Permeabilization
No
Specification
Lip
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde
Username

Dr. Kevin Egan

Verified customer

投稿 Mar 23 2018

Abreviews
Application
Flow Cytometry
Sample
Saccharomyces cerevisiae Cell (live yeast staining)
Specification
live yeast staining
Preparation
Cell harvesting/tissue preparation method: harvest cells and wash with 1XPBS
Sample buffer: 1XPBS
Fixation
no fixation
Permeabilization
No
Gating Strategy
HSV-tag expression
Username

Abcam user community

Verified customer

投稿 Aug 10 2012

Thank you for your patience in this matter. The product, ab9533 (HSV1 antibody), contains 0.05% sodium azide. Please don't hesitate to contact us again if you have further questions.

It is approx 275 ug/ml

The concentration of this product is 3 mg/ml.

This antibody has not been tested in WB, therefore we can't guarantee results. It will take Abcam approximately 7 days to get the antibody, followed by another 24 hours before it arrives in the US.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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