製品の概要

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab19354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
ELISA 1/100 - 1/500.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 関連性HSV is a peptide epitope tag, sequence QPELAPEDPED.
  • 別名
    • HSV epitope tag antibody
    • QPELAPEDPED tag antibody

Anti-HSV tag antibody 画像

  • ab19354 staining mouse embryonic brain tissue sections by IHC-P.  Sections were PFA fixed and permeabilized in 0.3% Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C.  The primary antibody was diluted 1/400 and incubated with the sample for 16 hours at 4°C.  A Cy3® conjugated donkey anti-goat antibody was used as the secondary.

    See Abreview

  • ab19354 staining HSV tag in the Human HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 5% BSA for 2 hours at 25°C. Samples were incubated with primary antibody (1/200 in PBS) for 16 hour at 4°C. A FITC-conjugated Donkey anti-Goat polyclonal(1/1000) was used as the secondary antibody.

    See Abreview

Anti-HSV tag antibody (ab19354) 使用論文

This product has been referenced in:
  • Wong FK  et al. Sustained Pax6 Expression Generates Primate-like Basal Radial Glia in Developing Mouse Neocortex. PLoS Biol 13:e1002217 (2015). IF . Read more (PubMed: 26252244) »
  • Padilla BE  et al. Endothelin-converting enzyme-1 regulates endosomal sorting of calcitonin receptor-like receptor and beta-arrestins. J Cell Biol 179:981-97 (2007). Flow Cyt, IP . Read more (PubMed: 18039931) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12% gel)
Sample Human Purified protein (kidney)
Specification kidney
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

投稿 Sep 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Sep 04 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Saccharomyces cerevisiae Cell (Live yeast staining on surface displayed protein)
Specification Live yeast staining on surface displayed protein
Preparation Cell harvesting/tissue preparation method: spin down and wash with water
Sample buffer: 1XPBS
Permeabilization No
Gating Strategy HSV expression positive
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Dr. Lei Chen

Verified customer

投稿 Aug 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Embryonic brain)
Specification Embryonic brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 0.3% Triton X 100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 29 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"