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Accession number: NM_000291.The HsPGK1 primers and probe can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells. PGK1 (3-phosphoglycerate kinase 1) is a highly expressed housekeeping gene located on the X chromosome. The primers and probe are located in exon 1, within 1 kb of the transcription start site. In most female cells the locus is enriched with both active (e.g. Histone H3 K18 acetylation, see image with ab1191). and heterochromatic (e.g. Histone H3 K9 tri methylation, see image with ab8898) histone modification marks, as one copy of the gene undergoes X chromosome inactivation. In male cells, this locus is highly enriched with histone modifications associated with active gene transcription whereas it only shows low levels of heterochromatic histone modifications.
Our Abpromise guarantee covers the use of ab83593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Real Time PCR||Use at an assay dependent concentration.
Suitable for the analysis of chromatin immunoprecipitated DNA using Taqman® real time PCR. The probe is labelled with FAM/TAMRA. Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product.
ab83593 has not yet been referenced specifically in any publications.