製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to HSPC142
  • アプリケーション
    適用あり: IHC-P, IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 175-225 of Human HSPC142 (NP_054892.2).

  • ポジティブ・コントロール
    • Hela whole cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab115017 was affinity purified using an epitope specific to HSPC142 immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab115017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36. component.
    • 配列類似性
      Belongs to the BABAM1 family.
    • ドメイン
      The VWFA-like region is similar to the VWFA domain. Its presence reveals similarities between the structure of the 19S proteasome and the BRCA1-A complexes.
    • 細胞内局在
      Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BABA1_HUMAN antibody
      • babam1 antibody
      • BRCA1 A complex subunit MERIT40 antibody
      • BRISC and BRCA1-A complex member 1 antibody
      • C19orf62 antibody
      • FLJ20571 antibody
      • HSPC142 antibody
      • hypothetical protein LOC29086 antibody
      • Mediator of Rap80 interactions and targeting 40 kDa antibody
      • Mediator of RAP80 interactions and targeting subunit of 40 kDa antibody
      • MERIT 40 antibody
      • MERIT40 antibody
      • NBA1 antibody
      • New component of the BRCA1 A complex antibody
      • New component of the BRCA1-A complex antibody
      • Uncharacterized protein C19orf62 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling HSPC142 with ab115017 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of HSPC142 by Western Blot of Immunprecipitate.
      anti-HSPC142, at 1 µg/ml, staining HSPC142 in Hela whole cell lysate immunoprecipitated using ab115017 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    参考文献

    ab115017 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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