This antibody gave a positive result in ELISA against the immunizing peptide (ab37546).
Not yet tested in other applications.
May play a role in cellular stress response.
Note=A chromosomal aberration involving HSPBAP1 has been found in a family with renal carcinoma. Translocation t(2;3)(q35;q21) with the putative pseudogene DIRC3. Produces an hybrid mRNA encoding a truncated HSPBAP1 lacking the first 36 amino acids.
ICC/IF image of ab36982 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab36982, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2 cells.However, this Fast-Track antibody is not yet fully characterised.This image represents inconclusive preliminary data.
Anti-HSPBAP1 antibody (ab36982) 使用論文
has not yet been referenced specifically in any publications.