製品の概要

  • 製品名Anti-HSPA4L antibody
    HSPA4L 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HSPA4L
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide, corresponding to a region within C terminal amino acids 720-769 (

    RNKDERYDHLDPTEMEKVEKCISDAMSWLNSKMNAQNKLSLTQDPVVKVS

    ) of human HSPA4L (NP_055093).

  • ポジティブ・コントロール
    • Fetal brain lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified by peptide affinity chromatography method.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab81221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 94 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性The HSPA4L gene, also known as Apg1 or Osp94, belongs to the HSP110 heat shock gene family, which includes three genes encoding highly conserved proteins. HSPA4L is expressed ubiquitously and predominantly in the testis.
  • 細胞内局在Cytoplasm; Nucleus. Note= May translocate to the nucleus after heat shock
  • 参照データベース
  • 別名
    • 94kDa antibody
    • AI461691 antibody
    • APG 1 antibody
    • Heat shock 70kDa protein 4 like antibody
    • MGC117843 antibody
    • MGC187594 antibody
    • Osmotic stress protein 94 antibody
    • Osp94 antibody
    • OTTMUSP00000027739 antibody
    see all

Anti-HSPA4L antibody 画像

  • Anti-HSPA4L antibody (ab81221) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 94 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

Anti-HSPA4L antibody (ab81221) 使用論文

ab81221 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Breast cell line)
Loading amount 200000 cells
Specification Breast cell line
Gel Running Conditions Reduced Denaturing (12.5 % gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
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投稿 Feb 09 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (breast cell line)
Total protein in input 3e+006 cells
Specification breast cell line
Immuno-precipitation step Protein A
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投稿 Jan 27 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (Breast cell line)
Specification Breast cell line
Fixative Methanol
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 10%
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投稿 Jan 27 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"