Anti-Hsp90 beta (phospho S226) 抗体 (ab63562)
Key features and details
- Rabbit polyclonal to Hsp90 beta (phospho S226)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Hsp90 beta (phospho S226) antibody
Hsp90 beta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Hsp90 beta (phospho S226) -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide corresponding to Human Hsp90 beta (phospho S226).
Database link: P08238 -
ポジティブ・コントロール
- NIH/3T3 cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63562の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (2) |
1/500 - 1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
|
ICC/IF |
1/500 - 1/1000.
|
特記事項 |
---|
WB
1/500 - 1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa). |
ICC/IF
1/500 - 1/1000. |
ターゲット情報
-
機能
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. -
配列類似性
Belongs to the heat shock protein 90 family. -
ドメイン
The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins. -
翻訳後修飾
Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity. -
細胞内局在
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
参照データベース
- Entrez Gene: 3326 Human
- Entrez Gene: 15516 Mouse
- Entrez Gene: 301252 Rat
- Omim: 140572 Human
- SwissProt: P08238 Human
- SwissProt: P11499 Mouse
- SwissProt: P34058 Rat
- Unigene: 509736 Human
see all -
別名
- 90 kda heat shock protein beta HSP90 beta antibody
- D6S182 antibody
- FLJ26984 antibody
see all
画像
-
All lanes : Anti-Hsp90 beta (phospho S226) antibody (ab63562) at 1/500 dilution
Lane 1 : extracts from A549 cells, treated
with serum (20%, 15mins)
Lane 2 : extracts from A549 cells, treated
with serum (20%, 15mins) with immunizing phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 83 kDa
Observed band size: 83 kDa -
ab63562 at 1/500 dilution staining NIH/3T3 cells, without (left) and with (right) immunizing peptide.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab63562 は 1 報の論文で使用されています。
- Wang H et al. Hsp90a forms a stable complex at the cilium neck for the interaction of signalling molecules in IGF-1 receptor signalling. J Cell Sci 128:100-8 (2015). PubMed: 25359884