製品の概要

  • 製品名Anti-Hsp90 beta antibody [MBH90B]
    Hsp90 beta 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MBH90B] to Hsp90 beta
  • アプリケーション適用あり: Flow Cyt, WB, IP, ICCmore details
  • 種交差性
    交差種: Mouse, Cow, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Pig, Non Human Primates
  • 免疫原

    Synthetic peptide: EEVHHGEEEVE, corresponding to amino acids 3-13 of Human Hsp90 beta

  • エピトープEEVHHG, corresponding to N terminal residues 3-8 of Hsp90b
  • ポジティブ・コントロール
    • MCF7 cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 0.2% high-grade protease free BSA, PBS
  • Concentration information loading...
  • 精製度>95% by SDS-PAGE
  • 特記事項(精製)Purified from ascites by protein-A affinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名MBH90B
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa). (under reducing conditions)
IP Use a concentration of 1 µg/ml.
ICC Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
  • 配列類似性Belongs to the heat shock protein 90 family.
  • ドメインThe TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.
  • 翻訳後修飾Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
    ISGylated.
    S-nitrosylated; negatively regulates the ATPase activity.
  • 細胞内局在Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 90 kda heat shock protein beta HSP90 beta antibody
    • D6S182 antibody
    • FLJ26984 antibody
    • Heat shock 84 kDa antibody
    • Heat shock 90kD protein 1, beta antibody
    • Heat shock 90kDa protein 1 beta antibody
    • Heat shock protein 90 alpha family class B member 1 antibody
    • Heat shock protein 90 kDa antibody
    • Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody
    • Heat shock protein 90kDa alpha family class B member 1 antibody
    • Heat shock protein beta antibody
    • Heat shock protein HSP 90 beta antibody
    • Heat shock protein HSP 90-beta antibody
    • HS90B_HUMAN antibody
    • HSP 84 antibody
    • HSP 90 antibody
    • HSP 90 b antibody
    • HSP 90b antibody
    • HSP84 antibody
    • HSP90 BETA antibody
    • hsp90ab1 antibody
    • HSP90B antibody
    • HSPC2 antibody
    • HSPCB antibody
    see all

Anti-Hsp90 beta antibody [MBH90B] 画像

  • Anti-Hsp90 beta antibody [MBH90B] (ab82395) at 1 µg/ml

    Predicted band size : 83 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HeLa cells stained with ab82395 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82395, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Hsp90 beta antibody [MBH90B] (ab82395) 使用論文

This product has been referenced in:
  • Barabutis N  et al. LPS induces pp60c-src-mediated tyrosine phosphorylation of Hsp90 in lung vascular endothelial cells and mouse lung. Am J Physiol Lung Cell Mol Physiol 304:L883-93 (2013). Mouse, Human . Read more (PubMed: 23585225) »
  • Joshi P & Stoddart CA Impaired infectivity of ritonavir-resistant HIV is rescued by heat shock protein 90AB1. J Biol Chem 286:24581-92 (2011). WB ; Human . Read more (PubMed: 21602280) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab82395.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"