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Full length native protein (purified) corresponding to Hsp90. Full length native protein (purified) (Achlya ambisexualis).
Our Abpromise guarantee covers the use of ab1429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.|
|IHC-Fr||Use a concentration of 10 - 20 µg/ml.|
|IP||Use a concentration of 4 - 8 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 90 kDa. Lysates should be heat-shocked to detect Hsp90.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 23209418|
Immunofluorescence analysis of human TIVE-L1 cells labeling HSP90 with ab1429 at 1:500 dilution. LANA was also labeled in red. Cells were fixed with 3% paraformaldehyde for 20mins, permeabilized with 0.2% Triton X-100 for 15mins and incubated in blocking buffer followed by the antibody. Slides were then incubated with the secondary antibody anti-mouse FITC. Nuclear staining with DAPI is shown in blue.
Overlay histogram showing HeLa cells stained with ab1429 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1429, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.