![Western blot - Hsp90 antibody [S88] (ab1429) image](#ab1429_2.jpg)
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Products:Signal Transduction >> Protein Trafficking >> Chaperones >> Heat Shock Proteins
Overview
Product name
Anti-Hsp90 antibody [S88]
Hsp90 抗体 (21件) 一覧
Description
Mouse monoclonal [S88] to Hsp90
Specificity
The antibody recognizes a 90 kDa protein, corresponding to the apparent molecular weight of Hsp90 on SDS-PAGE immunoblots. This antibody detects both Hsp90 alpha and beta.
Tested applications
IHC-Fr, IP, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Dog, Human, Pig, Monkey
Immunogen
Full length native protein (purified) (Achlya ambisexualis).
Positive control
Heated shocked HeLa cell lysate; Heated shocked 3T3 cell lysate; Heat shocked PC-12 cell lysate; Heat shocked CHO-K1 cell lysate; Heat shocked Rat-2 cell lysate; and recombinant Hsp90 protein
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
S88
Isotype
IgG1
Research areas
Cancer >> Tumor biomarkers >> Other
Signal Transduction >> Protein Trafficking >> Chaperones >> Heat Shock Proteins
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Western blot - Hsp90 antibody [S88] (ab1429)
![Western blot - Hsp90 antibody [S88] (ab1429) image](/ps/datasheet/Images/1/ab1429/ab1429_2.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab1429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use a concentration of 10 - 20 µg/ml.
IP: Use a concentration of 4 - 8 µg/ml.
WB: Use a concentration of 1 - 2 µg/ml.Predicted molecular weight: 90 kDa.(Lysates should be heat-shocked to detect Hsp90.)
Target
Relevance
In mammalian cells there are at least two Hsp90 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steriod receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways.
Cellular localization
Cytoplasmic
Alternative names
- D6S182 antibody
- FLJ26984 antibody
- FLJ31884 antibody
see all
Database links
- Entrez Gene: 396188 Chicken
- Entrez Gene: 281832 Cow
- Entrez Gene: 386588 Cow
- Entrez Gene: 3320 Human
- Entrez Gene: 3326 Human
- Entrez Gene: 15516 Mouse
- Entrez Gene: 15519 Mouse
- Entrez Gene: 396742 Pig
see all
Anti-Hsp90 antibody [S88] images:
Western blot - Hsp90 antibody [S88] (ab1429)
Anti-Hsp90 antibody [S88] (ab1429) + HeLa cell lysate
Predicted band size : 90 kDa
Observed band size : 90 kDa
References for Anti-Hsp90 antibody [S88] (ab1429)
This product has been referenced in:
- Cheng Jet al. 20-hydroxy-5,8,11,14-eicosatetraenoic acid mediates endothelial dysfunction via IkappaB kinase-dependent endothelial nitric-oxide synthase uncoupling. J Pharmacol Exp Ther 332:57-65 (2010). IP; Cow.Read more (PubMed: 19841472) »
- Wen KW & Damania B Hsp90 and Hsp40/Erdj3 are required for the expression and anti-apoptotic function of KSHV K1. Oncogene 29:3532-44 (2010). WB, IP; Human.Read more (PubMed: 20418907) »
See all 8 publications for this product
Publishing research using ab1429? Please let us know so that we can cite the reference in this datasheet
![Hsp90 antibody [S88] for WB in Human (1429)](/ps/datasheet/images/1/ab1429/Hsp90-Primary-antibodies-ab1429-1.jpg)
![Hsp90 antibody [S88] for WB in Human (1429)](/ps/Reviews/Images/ab6943_28968.jpg)
![Hsp90 antibody [S88] for WB in Human (1429)](/ps/Reviews/Images/ab7950_34633.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"