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Purified full length human Hsp90 beta
Our Abpromise guarantee covers the use of ab58950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).|
|IP||Use a concentration of 10 µg/ml.|
|ICC/IF||Use at an assay dependent concentration.
Cells were grown on sterile coverslips then fixed with 3.7% formaldehyde in PBS for 10 minutes and permeabilized with 0.2% Triton X-100 for 10 minutes at room temperature.
|Flow Cyt||Use 0.01-0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
Image from Niture SK et al, J Biol Chem. 2010 Nov 19;285(47):36865-75. Epub 2010 Sep 23, Fig 6. DOI 10.1074/jbc.M110.175802.Western Blot analysis of the effect of siRNA inhibition of Hsp90 on INrf2. Murine hepatoma (Hepa-1) cells were transfected with control siRNA or Hsp90-specific siRNA and Hsp90 (ab58950), INrf2, and actin levels were analyzed by immunoblotting.