製品の概要

  • 製品名Anti-Hsp90 antibody [AC88]
    Hsp90 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AC88] to Hsp90
  • 特異性Ab13492 recognises both Hsp90 alpha and beta. It reacts with the rodent glucocorticoid receptor and with proliferation potential proteins (P2PS) with apparent molecular masses of 30-40 kDa, which are associated with the 30-40S substructures of nuclear hnRNP complexes and share an epitope in common with Hsp90.
  • アプリケーション適用あり: IHC-Fr, IHC-P, ICC/IF, WB, IP, Flow Cyt, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Caenorhabditis elegans, Carp, Fish, Monkey, African Green Monkey, Rainbow Trout
  • 免疫原

    Achlya ambisexualis (water mold) Hsp90.

  • エピトープThe epitope of this antibody has been mapped to amino acid residues 604-697 of the human Hsp90 sequence.
  • ポジティブ・コントロール
    • HeLa Cell Lysate (Heat Shocked). Hsp90 Protein. HepG2 cells PC12 cells 3T3 cells Rat2 cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファーpH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)This antibody is affinity purified.
  • ポリ/モノモノクローナル
  • クローン名AC88
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab13492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 84.7 (alpha) , 83.2 (beta) kDa).
IP Use a concentration of 4 µg/ml. When used in immunoprecipitation, this antibody does not appear to react well with Hsp90 that is bound to steroid receptors or to pp60v-src of Rouse sarcoma virus.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC Use at an assay dependent concentration.

ターゲット情報

Anti-Hsp90 antibody [AC88] 画像

  • Anti-Hsp90 antibody [AC88] (ab13492) at 1/1000 dilution + Human fibroblast whole cell lysate at 40 µg

    Secondary
    HRP-conjugated goat anti-mouse polyclonal IgG at 1/2000 dilution
    Developed using the ECL technique

    Predicted band size : 84.7 (alpha) , 83.2 (beta) kDa


    Exposure time : 20 seconds

    This image is courtesy of an anonymous Abreview

    Blocked with 5% Milk for 2 hours at 22°C

    See Abreview

  • ab13492 staining Hsp90 (red) and another antibody to C2GnT-M (Golgi enzyme, greeen) in Panc-1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 1% Donkey serum in PBST) for 1 hour at 22°C. An undiluted DyLight® 594-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.

     

    See Abreview

  • ab13492 staining Hsp90 in Human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a TRIS-EDTA Buffer. Samples were incubated with primary antibody (1/500) for 30 minutes at 20°C. A HRP-conjugated Goat anti-rabbit/mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab13492 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13492, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ab13492 staining Hsp90 in Human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (10 ug/ml).

  • All lanes : Anti-Hsp90 antibody [AC88] (ab13492) at 1/1000 dilution

    Lane 1 : Hsp90 native human protein
    Lane 2 : Hsp90 beta reombinant human protein
    Lane 3 : Hsp90 alpha reombinant human protein
    Lane 4 : Cell lysates prepared from heat shocked Hela cells
    Lane 5 : Cell lysates prepared from heat shocked 3T3 cells
    Lane 6 : Cell lysates prepared from heat shocked PC-12 cells
    Lane 7 : Cell lysates prepared from heat shocked CHO-K1 cells
    Lane 8 : Cell lysates prepared from heat shocked Rat-2 cells


    Predicted band size : 84.7 (alpha) , 83.2 (beta) kDa
  • ICC/IF image of ab13492 stained HepG2 cells (ab7900). The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13492, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab13492 Immunoprecipitate OF hSP 90 in human AGS whole cell lysate. 200µg of cell lysate was incubated with primary antibody (1/250 in RIPA buffer) and matrix (Protein A/G) for 16 hours at 4°C.
    For western blotting anti-mouse HRP (ab6728) (1/1000) was used.

    See Abreview

Anti-Hsp90 antibody [AC88] (ab13492) 使用論文

This product has been referenced in:
  • Donet M  et al. Elastin peptides regulate HT-1080 fibrosarcoma cell migration and invasion through an Hsp90-dependent mechanism. Br J Cancer 111:139-148 (2014). Read more (PubMed: 24874477) »
  • Petrosyan A & Cheng PW Golgi fragmentation induced by heat shock or inhibition of heat shock proteins is mediated by non-muscle myosin IIA via its interaction with glycosyltransferases. Cell Stress Chaperones 19:241-54 (2014). WB, ICC/IF ; Human . Read more (PubMed: 23990450) »

See all 14 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Hamster Cell lysate - whole cell (CHO cells)
Gel Running Conditions Reduced Denaturing (8%)
Loading amount 20 µg
Treatment Infected with virus
Specification CHO cells
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Aug 24 2016

Application Immunohistochemistry (Frozen sections)
Blocking step 0.25%TritonX100, 0.2%Gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Sample Mouse Tissue sections (mouse embryonic spinal cord E11)
Specification mouse embryonic spinal cord E11
Permeabilization Yes - 0.25%TritonX100
Fixative Paraformaldehyde
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投稿 Aug 01 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Sample Human Tissue sections (Testis)
Specification Testis
Permeabilization Yes - Wash Buffer from Dako with Tween
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

投稿 Mar 31 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample Human Cell (Panc-1)
Specification Panc-1
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Nov 19 2013

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Sample Human Cell lysate - whole cell (Panc-1 cell lysate)
Specification Panc-1 cell lysate
Treatment 1-control 2- drug KNK437 35 mcm for 1h 3 - drug Brefeldin A 30 mcm for 30 min
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

投稿 Nov 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Cow Cell lysate - whole cell (oviductal epithelial cells)
Specification oviductal epithelial cells
Blocking step PVDF Blocking Reagent (TOYOBO) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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投稿 Sep 06 2013

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Antigen retrieval step None
Sample Apteronotus leptorhynchus Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde
Username

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Verified customer

投稿 Aug 29 2013

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Specification Brain
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Aug 29 2013



Ich kann bestätigen, dass wir diesen Antikörper in humaner Milz getestet haben.

Ein Kunde hat einen Abreview eingereicht der ein sehr schönes Färbungsmuster in Magen zeigt.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"