製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Hsp90
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 250-325 of Human Hsp90

  • ポジティブ・コントロール
    • Heat Shocked HeLa, 3T3 and Rat 2 Cell Lysates IF/ICC: HeLa cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab90555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000. Predicted molecular weight: 83 kDa.

ターゲット情報

画像

  • ICC/IF image of ab90555 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90555, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Hsp90 antibody (ab90555) at 1/1000 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : HeLa Cell Lysate (Heat Shocked)
    Lane 3 : 3T3 Cell Lysate (Heat Shocked)
    Lane 4 : Rat 2 Cell Lysate (Heat Shocked)

    Predicted band size: 83 kDa

参考文献

This product has been referenced in:
  • Goldlust IS  et al. Mouse model implicates GNB3 duplication in a childhood obesity syndrome. Proc Natl Acad Sci U S A 110:14990-4 (2013). Read more (PubMed: 23980137) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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