製品の概要

  • 製品名
    Anti-Hsp90 antibody [16F1]
    Hsp90 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [16F1] to Hsp90
  • 特異性
    ab13494 detects both the alpha and beta isoforms of Hsp90.
  • アプリケーション
    適用あり: IHC-P, ELISA, WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Carp, Drosophila melanogaster, Monkey, Zebrafish, Rainbow trout
    交差が予測される動物種: Plants
  • 免疫原

    Human Hsp90 purified from therapeutic orchiectomy specimens.

  • ポジティブ・コントロール
    • HeLa Cell Lysate (Heat Shocked). Hsp90 Protein.
  • 特記事項

    This antibody is highly recommended over other Hsp90 antibodies for use with samples of fish origin.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab13495 as a replacement.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    This product has been affinity purified.
  • ポリ/モノ
    モノクローナル
  • クローン名
    16F1
  • アイソタイプ
    IgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab13494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent dilution.
ELISA 1/5000 - 1/25000.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 83.2 (beta) , 84.5 (alpha) kDa).
IP Use at an assay dependent dilution.

ターゲット情報

画像

  • Lane 1 : Anti-Hsp90 antibody [16F1] (ab13494) at 1000
    Lane 2 : No primary

    Lane 1 : 15ug/lane HEK293 whole cell lysate
    Lane 2 : 15ug/lane HEK293 whole cell lysate

    Secondary
    Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735) at 1/1000 dilution

    Performed under reducing conditions.

    Predicted band size : 83.2 (beta) , 84.5 (alpha) kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Dr Alan Hendrick

    See Abreview

  • All lanes : Anti-Hsp90 antibody [16F1] (ab13494) at 1/1000 dilution

    Lane 1 : Molecular weight ladder
    Lane 2 : Hsp90 native protein
    Lane 3 : Hsp90 recombinant protein
    Lane 4 : Hsp90 alpha recombinant protein
    Lane 5 : heat shocked Hela cell lysates
    Lane 6 : L-929 cell lysates
    Lane 7 : Rat-2 cell lysates
    Lane 8 : RK-13 cell lysates


    Predicted band size : 83.2 (beta) , 84.5 (alpha) kDa

参考文献

This product has been referenced in:
  • Tawo R  et al. The Ubiquitin Ligase CHIP Integrates Proteostasis and Aging by Regulation of Insulin Receptor Turnover. Cell 169:470-482.e13 (2017). Read more (PubMed: 28431247) »
  • Jørgensen MM  et al. Potentials and capabilities of the Extracellular Vesicle (EV) Array. J Extracell Vesicles 4:26048 (2015). Read more (PubMed: 25862471) »

See all 12 Publications for this product

レビューと Q&A

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Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Sample
Mouse Cell lysate - whole cell (primary embryonic neurons)
Specification
primary embryonic neurons
Blocking step
Amersham blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 29 2014

Application
Immunocytochemistry/ Immunofluorescence
Sample
Fruit fly (Drosophila melanogaster) Tissue sections (Larval CNS)
Specification
Larval CNS
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS 0.1% triton
Username

Abcam user community

Verified customer

投稿 Oct 13 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (aortic endothelial)
Loading amount
5 µg
Specification
aortic endothelial
Treatment
Immunoprecipitated with AMPK
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Apr 28 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Lung)
Loading amount
20 µg
Specification
Lung
Treatment
TSA 1uM
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jan 14 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK-293 Cell Line)
Loading amount
15 µg
Specification
HEK-293 Cell Line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Username

Dr. Alan Hendrick

Verified customer

投稿 Oct 23 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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