製品の概要

  • 製品名Anti-Hsp90 antibody [16F1]
    Hsp90 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [16F1] to Hsp90
  • 特異性ab13494 detects both the alpha and beta isoforms of Hsp90.
  • アプリケーション適用あり: IHC-P, ELISA, WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Carp, Drosophila melanogaster, Monkey, Zebrafish, Rainbow Trout
    交差が予測される動物種: Plants
  • 免疫原

    Human Hsp90 purified from therapeutic orchiectomy specimens.

  • ポジティブ・コントロール
    • HeLa Cell Lysate (Heat Shocked). Hsp90 Protein.
  • 特記事項This antibody is highly recommended over other Hsp90 antibodies for use with samples of fish origin.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)This product has been affinity purified.
  • ポリ/モノモノクローナル
  • クローン名16F1
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab13494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent dilution.
ELISA 1/5000 - 1/25000.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 83.2 (beta) , 84.5 (alpha) kDa).
IP Use at an assay dependent dilution.

ターゲット情報

Anti-Hsp90 antibody [16F1] 画像

  • Lane 1 : Anti-Hsp90 antibody [16F1] (ab13494) at 1000
    Lane 2 : No primary

    Lane 1 : 15ug/lane HEK293 whole cell lysate
    Lane 2 : 15ug/lane HEK293 whole cell lysate

    Secondary
    Rabbit Anti-Rat IgG H&L (Alkaline Phosphatase) (ab6735) at 1/1000 dilution

    Performed under reducing conditions.

    Predicted band size : 83.2 (beta) , 84.5 (alpha) kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Dr Alan Hendrick

    See Abreview

  • All lanes : Anti-Hsp90 antibody [16F1] (ab13494) at 1/1000 dilution

    Lane 1 : Molecular weight ladder
    Lane 2 : Hsp90 native protein
    Lane 3 : Hsp90 recombinant protein
    Lane 4 : Hsp90 alpha recombinant protein
    Lane 5 : heat shocked Hela cell lysates
    Lane 6 : L-929 cell lysates
    Lane 7 : Rat-2 cell lysates
    Lane 8 : RK-13 cell lysates


    Predicted band size : 83.2 (beta) , 84.5 (alpha) kDa

Anti-Hsp90 antibody [16F1] (ab13494) 使用論文

This product has been referenced in:
  • Yoo JY  et al. Bortezomib-induced unfolded protein response increases oncolytic HSV-1 replication resulting in synergistic antitumor effects. Clin Cancer Res 20:3787-98 (2014). Read more (PubMed: 24815720) »
  • Taylor SY  et al. Folic acid modulates eNOS activity via effects on posttranslational modifications and protein-protein interactions. Eur J Pharmacol 714:193-201 (2013). Read more (PubMed: 23796957) »

See all 12 Publications for this product

Product Wall

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Sample Mouse Cell lysate - whole cell (primary embryonic neurons)
Specification primary embryonic neurons
Blocking step Amersham blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 29 2014

Thank you for contacting us. To our knowledge, tha antibody ab13494 has not been tested in flow cytometry. It may work for that application, on fixed and permeabilized samples, but we do not guarantee this. We do not have small samples for testing....

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I am very pleased to hear you would like to accept our offer and test ab13494 in fall armyworm (SF9 cells). This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for fall armyworm a...

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Thank you for contacting us. Unfortunately, none of the antibodies you mentioned (ab91259, ab83036, ab13492) have been tested in zebrafish so far. However, we have another HSP90 antibody that was tested in zebrafish: ab13494 (http://w...

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Application Immunocytochemistry/ Immunofluorescence
Sample Fruit fly (Drosophila melanogaster) Tissue sections (Larval CNS)
Specification Larval CNS
Fixative Paraformaldehyde
Permeabilization Yes - PBS 0.1% triton
Username

Abcam user community

Verified customer

投稿 Oct 13 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (aortic endothelial)
Loading amount 5 µg
Specification aortic endothelial
Treatment Immunoprecipitated with AMPK
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Apr 28 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Lung)
Loading amount 20 µg
Specification Lung
Treatment TSA 1uM
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jan 14 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK-293 Cell Line)
Loading amount 15 µg
Specification HEK-293 Cell Line
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Username

Dr. Alan Hendrick

Verified customer

投稿 Oct 23 2006

Thank you for contacting us for technical support, I'm sorry to hear you are experiencing problems with ab13494. I think the fact that another antibody against Hsp90 worked well rules out the possibility that your samples express low levels of the ...

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Thank you for your enquiry. I have added references for the antibody as used in IHC to the datasheet. You may refer to these articles for further information. I have included a link to the datasheet below for your convenience. I hope this infor...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"