製品の概要

  • 製品名Anti-Hsp90 alpha antibody [EPR3953]
    Hsp90 alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3953] to Hsp90 alpha
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to region within Human Hsp90 alpha.

  • ポジティブ・コントロール
    • WB: HeLa and 293T cell lysates IHC-P: Human gastric adenocarcinoma tissue and Human kidney tissue IF: HeLa cells
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab109248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Molecular chaperone. Has ATPase activity.
    • 配列類似性Belongs to the heat shock protein 90 family.
    • 翻訳後修飾ISGylated.
    • 細胞内局在Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 参照データベース
    • 別名
      • EL52 antibody
      • epididymis luminal secretory protein 52 antibody
      • Heat shock 86 kDa antibody
      • heat shock 90kD protein 1, alpha antibody
      • Heat shock 90kD protein 1, alpha like 4 antibody
      • heat shock 90kD protein, alpha-like 4 antibody
      • Heat shock 90kDa protein 1 alpha antibody
      • Heat shock protein 90kDa alpha (cytosolic) class A member 1 antibody
      • Heat shock protein HSP 90-alpha antibody
      • HS90A_HUMAN antibody
      • HSP 86 antibody
      • HSP86 antibody
      • Hsp89 antibody
      • HSP89A antibody
      • Hsp90 antibody
      • HSP90A antibody
      • HSP90AA1 antibody
      • HSP90ALPHA antibody
      • HSP90N antibody
      • HSPC1 antibody
      • HSPCA antibody
      • HSPCAL1 antibody
      • HSPCAL4 antibody
      • HSPN antibody
      • LAP 2 antibody
      • LAP2 antibody
      • lipopolysaccharide-associated protein 2 antibody
      • LPS-associated protein 2 antibody
      • Renal carcinoma antigen NY-REN-38 antibody
      see all

    Anti-Hsp90 alpha antibody [EPR3953] 画像

    • All lanes : Anti-Hsp90 alpha antibody [EPR3953] (ab109248) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 85 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    • ab109248, at 1/100, staining Hsp90 alpha in paraffin-embedded Human gastric adenocarcinoma tissue by Immunohistochemistry.
    • ab109248, at 1/100, staining Hsp90 alpha in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • ab109248, at 1/100, staining Hsp90 alpha in HeLa cells by Immunofluorescence.

    Anti-Hsp90 alpha antibody [EPR3953] (ab109248) 使用論文

    This product has been referenced in:
    • Guo W  et al. Targeting GRP75 improves HSP90 inhibitor efficacy by enhancing p53-mediated apoptosis in hepatocellular carcinoma. PLoS One 9:e85766 (2014). IHC ; Human . Read more (PubMed: 24465691) »
    • Guarani V  et al. TIMMDC1/C3orf1 functions as a membrane-embedded mitochondrial complex I assembly factor through association with the MCIA complex. Mol Cell Biol 34:847-61 (2014). Read more (PubMed: 24344204) »

    See all 2 Publications for this product

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    There are currently no Abreviews or Questions for ab109248.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"