製品の概要

  • 製品名Anti-Hsp90 alpha antibody [4F10]
    Hsp90 alpha 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4F10] to Hsp90 alpha
  • アプリケーション適用あり: ELISA, WB, IHC-P, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant human Hsp90 (1-732aa) purified from E. coli.

  • ポジティブ・コントロール
    • HeLa lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab48022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
WB 1/1000 - 1/2000. Predicted molecular weight: 86 kDa.
IHC-P 1/100 - 1/200.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ターゲット情報

  • 機能Molecular chaperone. Has ATPase activity.
  • 配列類似性Belongs to the heat shock protein 90 family.
  • 翻訳後修飾ISGylated.
  • 細胞内局在Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EL52 antibody
    • epididymis luminal secretory protein 52 antibody
    • Heat shock 86 kDa antibody
    • heat shock 90kD protein 1, alpha antibody
    • Heat shock 90kD protein 1, alpha like 4 antibody
    • heat shock 90kD protein, alpha-like 4 antibody
    • Heat shock 90kDa protein 1 alpha antibody
    • Heat shock protein 90kDa alpha (cytosolic) class A member 1 antibody
    • Heat shock protein HSP 90-alpha antibody
    • HS90A_HUMAN antibody
    • HSP 86 antibody
    • HSP86 antibody
    • Hsp89 antibody
    • HSP89A antibody
    • Hsp90 antibody
    • HSP90A antibody
    • HSP90AA1 antibody
    • HSP90ALPHA antibody
    • HSP90N antibody
    • HSPC1 antibody
    • HSPCA antibody
    • HSPCAL1 antibody
    • HSPCAL4 antibody
    • HSPN antibody
    • LAP 2 antibody
    • LAP2 antibody
    • lipopolysaccharide-associated protein 2 antibody
    • LPS-associated protein 2 antibody
    • Renal carcinoma antigen NY-REN-38 antibody
    see all

Anti-Hsp90 alpha antibody [4F10] 画像

  • Anti-Hsp90 alpha antibody [4F10] (ab48022) at 1/1000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 86 kDa
    Observed band size : 86 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
  • ab48022 staining Hsp90 alpha in human urothelium tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer. The primary antibody was used at 1/100 dilution and incubated with sample for 2 hours at 24°C. The DAB polymer detection system was used with a DAB chromogen.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling HSP90 with ab48022 at 1/100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

  • Flow Cytometry analysis of HepG2 cells labelling HSP90 with ab48022, staining at 2-5 µg/1x106 cells (red). A 488-conjugated goat anti-mouse IgG was used as the secondary antibody. Black - Isotype control, mouse IgG.

Anti-Hsp90 alpha antibody [4F10] (ab48022) 使用論文

ab48022 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab48022.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"