製品の概要

  • 製品名
    Anti-Hsp90 alpha antibody [4F10]
    Hsp90 alpha 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4F10] to Hsp90 alpha
  • アプリケーション
    適用あり: ELISA, WB, IHC-P, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant human Hsp90 (1-732aa) purified from E. coli.

  • ポジティブ・コントロール
    • HeLa lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab48022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
WB 1/1000 - 1/2000. Predicted molecular weight: 86 kDa.
IHC-P 1/100 - 1/200.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Molecular chaperone. Has ATPase activity.
  • 配列類似性
    Belongs to the heat shock protein 90 family.
  • 翻訳後修飾
    ISGylated.
  • 細胞内局在
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EL52 antibody
    • epididymis luminal secretory protein 52 antibody
    • Heat shock 86 kDa antibody
    • heat shock 90kD protein 1, alpha antibody
    • Heat shock 90kD protein 1, alpha like 4 antibody
    • heat shock 90kD protein, alpha-like 4 antibody
    • Heat shock 90kDa protein 1 alpha antibody
    • Heat shock protein 90kDa alpha (cytosolic) class A member 1 antibody
    • Heat shock protein HSP 90-alpha antibody
    • HS90A_HUMAN antibody
    • HSP 86 antibody
    • HSP86 antibody
    • Hsp89 antibody
    • HSP89A antibody
    • Hsp90 antibody
    • HSP90A antibody
    • HSP90AA1 antibody
    • HSP90ALPHA antibody
    • HSP90N antibody
    • HSPC1 antibody
    • HSPCA antibody
    • HSPCAL1 antibody
    • HSPCAL4 antibody
    • HSPN antibody
    • LAP 2 antibody
    • LAP2 antibody
    • lipopolysaccharide-associated protein 2 antibody
    • LPS-associated protein 2 antibody
    • Renal carcinoma antigen NY-REN-38 antibody
    see all

画像

  • Anti-Hsp90 alpha antibody [4F10] (ab48022) at 1/1000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 86 kDa
    Observed band size : 86 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
  • ab48022 staining Hsp90 alpha in human urothelium tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer. The primary antibody was used at 1/100 dilution and incubated with sample for 2 hours at 24°C. The DAB polymer detection system was used with a DAB chromogen.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling HSP90 with ab48022 at 1/100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

  • Flow Cytometry analysis of HepG2 cells labelling HSP90 with ab48022, staining at 2-5 µg/1x106 cells (red). A 488-conjugated goat anti-mouse IgG was used as the secondary antibody. Black - Isotype control, mouse IgG.

参考文献

ab48022 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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