製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Hsp70
  • 特異性
    Detects a 70kDa protein corresponding to the molecular mass of inducible hsp70. May cross-react with Hsc70 at lower dilutions.
  • アプリケーション
    適用あり: IHC-Fr, ICC/IF, WB, IP, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human, Orangutan
    交差が予測される動物種: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Macaque monkey, African green monkey, Chinese hamster, Bos mutus grunniens, Saguinus oedipus
  • 免疫原

    Full length protein corresponding to Human Hsp70 aa 1-641.
    Sequence:

    MAKAAAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERL IGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDK PKVQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYF NDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDL GGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHK KDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRA RFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLL QDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLS LGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMT KDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANK ITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMK SAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELE QVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD


    Database link: P08107

  • ポジティブ・コントロール
    • HeLa, A431, A549, HCT116, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3, T98G and Rat Braiin tumour lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/100.
WB 1/10000. Detects a band of approximately 70 kDa.
IP 1/100.
IHC-P 1/100.
ELISA Use at an assay dependent concentration.

ターゲット情報

Anti-Hsp70 antibody 画像

  • All lanes : Anti-Hsp70 antibody (ab79852) at 1/25000 dilution

    Lane 1 : Cell lysates prepared from mouse Pam212 cells
    Lane 2 : Molecular weight marker
  • Immunohistochemistry of Human colon carcinoma staining Hsp70 using ab79852 at 1:50000. A Biotin conjugated Goat Anti-Rabbit antibody at 1:2000 was used as a secondary antibody and Methyl Green at 200uL was used to counter stain.

  • Immunocytochemistry/Immunofluorescence analysis of Heat Shocked HeLa Cells staining Hsp70 using ab79852 at a 1:100 dilution. The secondary antibody was a FITC conjugated Goat Anti-Rabbit (green) at a 1:200 dilution. Counterstain: DAPI (blue) nuclear stain at 1:40000. A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite.

  • ab79852 staining Hsp70 in Mouse backskin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

  • All lanes : Anti-Hsp70 antibody (ab79852) at 1/25000 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : Cell lysates prepared from human A431 cells
    Lane 3 : Cell lysates prepared from human A549 cells
    Lane 4 : Cell lysates prepared from human HCT116 cells
    Lane 5 : Cell lysates prepared from Hela cells
    Lane 6 : Cell lysates prepared from HEK293 cells
    Lane 7 : Cell lysates prepared from HepG2 cells
    Lane 8 : Cell lysates prepared from HL-60 cells
    Lane 9 : Cell lysates prepared from HUVEC cells
    Lane 10 : Cell lysates prepared from Jurkat cells
    Lane 11 : Cell lysates prepared from MCF7 cells
    Lane 12 : Cell lysates prepared from PC3 cells
    Lane 13 : Cell lysates prepared from T98G cells
    Lane 14 : Tissue lysates prepared from Rat brain

Anti-Hsp70 antibody (ab79852) 使用論文

This product has been referenced in:
  • Yang X  et al. The histological characteristics, age-related thickness change of skin, and expression of the HSPs in the skin during hair cycle in yak (Bos grunniens). PLoS One 12:e0176451 (2017). WB, IHC ; Other . Read more (PubMed: 28463974) »
  • Sokolina K  et al. Systematic protein-protein interaction mapping for clinically relevant human GPCRs. Mol Syst Biol 13:918 (2017). IP . Read more (PubMed: 28298427) »

See all 9 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
gelatin in KPBS as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 21°C
Sample
Mouse Cell (Quadriceps Tissue)
Specification
Quadriceps Tissue
Permeabilization
No
Fixative
none
Username

Abcam user community

Verified customer

投稿 Jan 07 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (HUVEC)
Specification
HUVEC
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative
Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

投稿 Nov 21 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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