Anti-Hsp70 抗体 (ab31010)
Key features and details
- Rabbit polyclonal to Hsp70
- Suitable for: IHC-P, IP, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Hsp70 antibody
Hsp70 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Hsp70 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig -
免疫原
Synthetic peptide within Human Hsp70 aa 550 to the C-terminus. The exact sequence is proprietary.
Database link: P0DMV8 -
ポジティブ・コントロール
- Hela cells. Breast cancer.
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特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab31010 is an affinity purified IgG. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31010の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | ||
IP | ||
WB | (1) |
特記事項 |
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citrate buffer, pH 6.0 for 10 min and then cool at RT for 20 min.
WB: Use at an assay dependent dilution. Predicted molecular weight: 70 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性
Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific. -
細胞内局在
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. -
参照データベース
- Entrez Gene: 281825 Cow
- Entrez Gene: 403612 Dog
- Entrez Gene: 15511 Human
- Entrez Gene: 3303 Human
- Entrez Gene: 193740 Mouse
- Entrez Gene: 3304 Mouse
- Entrez Gene: 396906 Pig
- Entrez Gene: 24472 Rat
see all -
別名
- DnaK type molecular chaperone HSP70 1 antibody
- Epididymis secretory protein Li 103 antibody
- FLJ54303 antibody
see all
画像
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Hsp70 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Hsp70 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31010.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 70kDa; Hsp70
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Tissue: Human breast cancer (formalin fixed/paraffin).
Staining: ab31010 at 1/100 dilution for 30 minutes at room temperature. -
Anti-Hsp70 antibody (ab31010) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 70 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Additional bands at: 76 kDa (possible post-translational modification) -
Anti-Hsp70 antibody (ab31010) at 1/25 dilution + 293 cell lysate
Predicted band size: 70 kDa
Observed band size: 70 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab31010 は 13 報の論文で使用されています。
- Yang J et al. Exosomal miR-638 Inhibits Hepatocellular Carcinoma Progression by Targeting SP1. Onco Targets Ther 13:6709-6720 (2020). PubMed: 32764961
- Scalabrin M et al. Redox responses in skeletal muscle following denervation. Redox Biol 26:101294 (2019). PubMed: 31450104
- Yang X et al. LncRNA MALAT1 shuttled by bone marrow-derived mesenchymal stem cells-secreted exosomes alleviates osteoporosis through mediating microRNA-34c/SATB2 axis. Aging (Albany NY) 11:8777-8791 (2019). PubMed: 31659145
- Zhao ZW et al. Heat shock protein 70 accelerates atherosclerosis by downregulating the expression of ABCA1 and ABCG1 through the JNK/Elk-1 pathway. Biochim Biophys Acta 1863:806-822 (2018). WB . PubMed: 29678642
- Yeung HY et al. Anti-tumor immunity of BAM-SiPc-mediated vascular photodynamic therapy in a BALB/c mouse model. Cell Mol Immunol 14:223-234 (2017). PubMed: 26388236