製品の概要

  • 製品名Anti-Hsp70 antibody
    Hsp70 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Hsp70
  • 特異性This antibody reacts with the two major human HSP 70s (HSP 72 and HSP 73). It stains epithelial cells in many different tissues e.g. tonsil, stomach, small and large intestine, bile ducts, ovary and prostate.
  • アプリケーション適用あり: IHC-P, IHC-Fr, WBmore details
    適用なし: ELISA
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Purified heat shock protein (Dnak) from E. coli.

  • ポジティブ・コントロール
    • Breast carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/75 - 1/250. Use an ABC method and incubate for 30 minutes at room temperature.
IHC-Fr 1/75 - 1/250. Use an ABC method and incubate for 30 minutes at room temperature.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 70 kDa).
  • 追加情報Is unsuitable for ELISA.
  • ターゲット情報

    • 機能In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
    • 組織特異性HSPA1B is testis-specific.
    • 配列類似性Belongs to the heat shock protein 70 family.
    • 細胞内局在Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DnaK type molecular chaperone HSP70 1 antibody
      • Epididymis secretory protein Li 103 antibody
      • FLJ54303 antibody
      • FLJ54370 antibody
      • FLJ54392 antibody
      • FLJ54408 antibody
      • FLJ75127 antibody
      • Heat shock 70 kDa protein 1 antibody
      • Heat shock 70 kDa protein 1/2 antibody
      • Heat shock 70 kDa protein 1A/1B antibody
      • Heat shock 70kDa protein 1A antibody
      • Heat shock 70kDa protein 1B antibody
      • Heat shock induced protein antibody
      • HEL S 103 antibody
      • HSP70 1 antibody
      • HSP70 1B antibody
      • HSP70 2 antibody
      • HSP70-1/HSP70-2 antibody
      • HSP70-1A antibody
      • HSP70.1 antibody
      • HSP70.1/HSP70.2 antibody
      • HSP70I antibody
      • HSP71_HUMAN antibody
      • HSP72 antibody
      • HSPA1 antibody
      • HSPA1A antibody
      • HSPA1B antibody
      see all

    Anti-Hsp70 antibody 画像

    • ab2412 staining Hsp70 in human breast carcinoma cells
    • All lanes : Anti-Hsp70 antibody (ab2412) at 1 µg/ml

      Lane 1 : Heart (Rat) Tissue Lysate
      Lane 2 : Spleen (Rat) Tissue Lysate
      Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size : 70 kDa
      Observed band size : 72 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue, staining Hsp70 with ab2412.

    Anti-Hsp70 antibody (ab2412) 使用論文

    ab2412 has not yet been referenced specifically in any publications.

    Product Wall

    I received this morning the following information from the source of ab2412: "we have not seen any drop in the quality of ab2412 in IHC, neither have we heard any complaints for lot # E676. We have totally exhausted lot # E392 and in fact have only o...

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    I received feedback from the laboratory that tested ab2412 in immunohistochemistry who confirmed there are no quality concerns with lot 165620 following those tests. As the antibody has not been tested in ELISA we cannot tell whether another lot would...

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    Thank you for contacting us for technical support regarding ab2412. To our knowledge this antibody had not been tested in ELISA so it is good to know that you have had good results with this antibody in the past. Currently the ELISA application is no...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"