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Recombinant fragment, corresponding to amino acids 1-641 of Human Hsp70, purified from E. coli
Our Abpromise guarantee covers the use of ab48515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).|
|ELISA||Use at an assay dependent concentration.|
ab48515 staining Hsp70 in human eccrine gland section, by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer. The primary antibody was used at 1/100 dilution and incubated with sample for 2 hours at 24°C. The DAB detection system was used with DAB chromogen.
ab48515 has not yet been referenced specifically in any publications.