製品の概要

  • 製品名Anti-Hsp70 antibody [4E7]
    Hsp70 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4E7] to Hsp70
  • 特異性This antibody is specific for Hsp70
  • アプリケーション適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-641 of Human Hsp70, purified from E. coli

  • ポジティブ・コントロール
    • HeLa cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 10% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)The antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名4E7
  • ミエローマSp2/0
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab48515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100.
WB 1/1000 - 1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
  • 組織特異性HSPA1B is testis-specific.
  • 配列類似性Belongs to the heat shock protein 70 family.
  • 細胞内局在Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DnaK type molecular chaperone HSP70 1 antibody
    • Epididymis secretory protein Li 103 antibody
    • FLJ54303 antibody
    • FLJ54370 antibody
    • FLJ54392 antibody
    • FLJ54408 antibody
    • FLJ75127 antibody
    • Heat shock 70 kDa protein 1 antibody
    • Heat shock 70 kDa protein 1/2 antibody
    • Heat shock 70 kDa protein 1A/1B antibody
    • Heat shock 70kDa protein 1A antibody
    • Heat shock 70kDa protein 1B antibody
    • Heat shock induced protein antibody
    • HEL S 103 antibody
    • HSP70 1 antibody
    • HSP70 1B antibody
    • HSP70 2 antibody
    • HSP70-1/HSP70-2 antibody
    • HSP70-1A antibody
    • HSP70.1 antibody
    • HSP70.1/HSP70.2 antibody
    • HSP70I antibody
    • HSP71_HUMAN antibody
    • HSP72 antibody
    • HSPA1 antibody
    • HSPA1A antibody
    • HSPA1B antibody
    see all

Anti-Hsp70 antibody [4E7] 画像

  • Anti-Hsp70 antibody [4E7] (ab48515) at 1/1000 dilution + HeLa cell lysate (30µg)

    Secondary
    goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 70 kDa
    Observed band size : 70 kDa
  • ab48515 staining Hsp70 in human eccrine gland section, by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in 0.1M sodium citrate buffer. The primary antibody was used at 1/100 dilution and incubated with sample for 2 hours at 24°C. The DAB detection system was used with DAB chromogen.

Anti-Hsp70 antibody [4E7] (ab48515) 使用論文

ab48515 has not yet been referenced specifically in any publications.

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