Anti-Hsp60 抗体 [LK-2] (ab59458)
Key features and details
- Mouse monoclonal [LK-2] to Hsp60
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Hsp60 antibody [LK-2]
Hsp60 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [LK-2] to Hsp60 -
由来種
Mouse -
特異性
Detects ~60kDa.
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アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Human Hsp60 produced through recombinant DNA methods in E.coli
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ポジティブ・コントロール
- HeLa cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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ポリ/モノ
モノクローナル -
クローン名
LK-2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59458の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100.
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WB | (1) |
1/40000. Predicted molecular weight: 60 kDa.
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特記事項 |
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IHC-P
1/100. |
WB
1/40000. Predicted molecular weight: 60 kDa. |
ターゲット情報
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機能
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. -
関連疾患
Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life. -
配列類似性
Belongs to the chaperonin (HSP60) family. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 3329 Human
- Entrez Gene: 15510 Mouse
- Omim: 118190 Human
- SwissProt: P10809 Human
- SwissProt: P63038 Mouse
- Unigene: 595053 Human
- Unigene: 727543 Human
- Unigene: 1777 Mouse
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別名
- 60 kDa chaperonin antibody
- 60 kDa heat shock protein, mitochondrial antibody
- CH60_HUMAN antibody
see all
画像
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Immunohistochemistry analysis of Paraffin Embedded Human kidney tissue using Mouse Anti-HSP60 Antibody, Clone LK2 (SMC-111) at 1/100 dilution.
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Anti-Hsp60 antibody [LK-2] (ab59458) at 1/1000 dilution + HeLa cell lysate
Predicted band size: 60 kDa -
Lanes 1-3 : Anti-Hsp60 antibody [LK-2] (ab59458) at 1/4000 dilution
Lanes 1-3 : Saccharomyces cerevisiae whole cell lysate at 6900000 cells
Lane 4 : Ladder
Secondary
Lanes 1-2 : HRP-conjugated goat anti-mouse IgG monoclonal at 1/5000 dilution
Lane 3 : HRP-conjugated goat anti-mouse IgG monoclonal at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 25 seconds
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ab59464 at 100,000 dilution staining Hsp60 alpha in human colon tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ system was used for staining. A HRP-conjugated anti mouse secondary was used at 1/10 dilution.
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Paraffin-embedded mouse back skin tissue stained for Hsp60 using ab59458 at 1/100 dilution in immunohistochemical analysis. DAB staining.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab59458 は 6 報の論文で使用されています。
- Jiang X et al. Exploiting genetic diversity and gene synthesis to identify superior nitrogenase NifH protein variants to engineer N2-fixation in plants. Commun Biol 4:4 (2021). PubMed: 33398015
- Senger F et al. Spatial integration of mechanical forces by a-actinin establishes actin network symmetry. J Cell Sci 132:N/A (2019). PubMed: 31615968
- Farrugia G et al. Aspirin impairs acetyl-coenzyme A metabolism in redox-compromised yeast cells. Sci Rep 9:6152 (2019). PubMed: 30992471
- Schultz MJ et al. Variable Inhibition of Zika Virus Replication by Different Wolbachia Strains in Mosquito Cell Cultures. J Virol 91:N/A (2017). PubMed: 28446677
- Mion F et al. Mast cells control the expansion and differentiation of IL-10-competent B cells. J Immunol 193:4568-79 (2014). PubMed: 25267976
- Bivi N et al. Transcriptome and proteome analysis of osteocytes treated with nitrogen-containing bisphosphonates. J Proteome Res 8:1131-42 (2009). PubMed: 19226166