Anti-Hsp60 抗体 (ab31115)
Key features and details
- Rabbit polyclonal to Hsp60
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Hsp60 antibody
Hsp60 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Hsp60 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken, Cow, Pufferfish, Zebrafish, Sea urchin, Chinese hamster -
免疫原
Synthetic peptide within Human Hsp60 aa 1-100. The exact sequence is proprietary.
Database link: P10809 -
ポジティブ・コントロール
- Hela cells, NIH3T3 cells, Colon carcinoma.
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特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31115の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
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IHC-P |
1/200.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 60 kDa. |
IHC-P
1/200. |
ターゲット情報
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機能
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. -
関連疾患
Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life. -
配列類似性
Belongs to the chaperonin (HSP60) family. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 424059 Chicken
- Entrez Gene: 100689473 Chinese hamster
- Entrez Gene: 511913 Cow
- Entrez Gene: 3329 Human
- Entrez Gene: 15510 Mouse
- Entrez Gene: 63868 Rat
- Omim: 118190 Human
- SwissProt: Q5ZL72 Chicken
see all -
別名
- 60 kDa chaperonin antibody
- 60 kDa heat shock protein, mitochondrial antibody
- CH60_HUMAN antibody
see all
画像
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All lanes : Anti-Hsp60 antibody (ab31115) at 1/8000 dilution
Lanes 1 & 5 : Cow normal mammary epithelial cells (nMEC) treated with serum free media
Lanes 2 & 6 : Cow normal mammary epithelial cells (nMEC) treated with 1.0µM anisomycin
Lanes 3 & 7 : Cow normal mammary epithelial cells (nMEC) treated with 100ng/ml IGF-1
Lanes 4 & 8 : Cow normal mammary epithelial cells (nMEC) treated with anisomycin and IGF-1
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : ECL Anti-Rabbit IgG, Horseradish Peroxidase Linked at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 15 secondsTreatment for 30 minutes (lanes 1-4) or 1 hour (lanes 5-8). 12.5% SDS-PAGE. Proteins collected in RIPA Buffer and transferred to PVDF (0.45 um).
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Ab31115, at a 1/200 dilution, staining Hsp60 in formalin fixed, paraffin embedded human colon carcinoma tissue by Immunohistochemistry.
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Anti-Hsp60 antibody (ab31115) at 1/25 dilution + lysate prepared from HeLa cells
Predicted band size: 60 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab31115 は 6 報の論文で使用されています。
- Agostini-Dreyer A et al. IGFBP-3 Induced by Ribotoxic Stress Traffics From the Endoplasmic Reticulum to the Nucleus in Mammary Epithelial Cells. J Endocr Soc 3:517-536 (2019). PubMed: 30788454
- Jetzt AE et al. Toxicity of ricin A chain is reduced in mammalian cells by inhibiting its interaction with the ribosome. Toxicol Appl Pharmacol 310:120-128 (2016). PubMed: 27639428
- Vale-Costa S et al. Influenza A virus ribonucleoproteins modulate host recycling by competing with Rab11 effectors. J Cell Sci 129:1697-710 (2016). PubMed: 26940915
- Agostini-Dreyer A et al. Endogenous IGFBP-3 mediates intrinsic apoptosis through modulation of Nur77 phosphorylation and nuclear export. Endocrinology N/A:en20151215 (2015). PubMed: 26340041
- Li XS et al. Heat shock protein 60 overexpression is associated with the progression and prognosis in gastric cancer. PLoS One 9:e107507 (2014). IHC-P ; Human . PubMed: 25207654
- Jetzt AE et al. Ricin A-chain requires c-Jun N-terminal kinase to induce apoptosis in nontransformed epithelial cells. Int J Biochem Cell Biol 41:2503-10 (2009). WB ; Cow . PubMed: 19695342